Molecular cloning and characterization of a cDNA encoding proline transporter in rice
Molecular cloning and characterization of a cDNA encoding proline transporter in rice
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DOI：
10.1093/pcp/41.6.750
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发表时间：
2000-06-01
影响因子：
4.9
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
从水稻14龄苗的cDNA文库中分离到一个编码脯氨酸（Pro）转运蛋白（ProT）的cDNA。Akibare。推断的水稻ProT蛋白（OsProT）氨基酸序列与拟南芥（Arabidopsis thaliana）的ProT蛋白1同源性为68.8%，与番茄（lycopersicicon esculentum）的同源性为59.6%。Northern blot分析显示，OsProT基因（OsProT）在所有器官中均有表达，在叶鞘和茎中表达较强。盐处理没有诱导OsProT的表达，但强烈诱导了Pro生物合成的关键酶- δ(1)-吡啶-5-羧酸合成酶（P5CS）基因的表达。Southern blot分析显示OsProT有一个基因家族。在使用非洲爪蟾卵母细胞的运输试验中，OsProT特异性地运输L-Pro。
A cDNA encoding a proline (Pro) transporter (ProT) was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the rice ProT protein (OsProT) had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculentum. Northern blot analysis revealed that the gene for OsProT (OsProT) was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported L-Pro in a transport assay using Xenopus laevis oocytes.