Thyroid fetal male microchimerisms in mothers with thyroid disorders:: Presence of Y-chromosomal immunofluorescence in thyroid-infiltrating lymphocytes is more prevalent in Hashimoto's thyroiditis and Graves' disease than in follicular adenomas
Thyroid fetal male microchimerisms in mothers with thyroid disorders:: Presence of Y-chromosomal immunofluorescence in thyroid-infiltrating lymphocytes is more prevalent in Hashimoto's thyroiditis and Graves' disease than in follicular adenomas
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DOI：
10.1210/jc.2004-1049
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发表时间：
2004-11-01
影响因子：
5.8
通讯作者：
Badenhoop, K
中科院分区：
文献类型：
--
作者：
Renné, C;Lopez, ER;Badenhoop, K
母体隔室中胎儿细胞的存在被定义为胎儿-母体微嵌合体，其已在患有自身免疫的母亲的甲状腺中检测到。我们分析了49例桥本甲状腺炎（n=25）、Graves病（n=15）或结节性或弥漫性滤泡性腺瘤（n=9）患者手术时石蜡包埋甲状腺标本的免疫组织学，这些患者的分娩史为男孩阳性。通过荧光原位杂交，我们筛选了X染色体和Y染色体特异性染色，并将发现与人类白细胞抗原（HLA）DQ类型的母亲和他们的后代（如果可用）进行比较。在23例甲状腺中，我们发现Y染色体特异性染色，这是更常见的甲状腺自身免疫性疾病（60%桥本甲状腺炎和40%格雷夫斯病）比滤泡性腺瘤（22.2%）。甲状腺Y染色体染色组与非Y染色体染色组间HLA DQ等位基因无显著性差异。然而，所有研究的微嵌合体阳性母子对和患有桥本甲状腺炎和Graves病的妇女中的一个亚组更常具有易感等位基因HLA DQA 1 * 0501-DQB 1 *0201或DQB 1 *0301。总之，胎儿微嵌合体是在母亲的甲状腺与儿子，这是发现更频繁的甲状腺自身免疫性疾病。
The presence of fetal cells in a maternal compartment is defined as fetal-maternal microchimerism, which has been detected in thyroids of mothers suffering from autoimmunity. We analyzed the immunohistology of paraffin-embedded thyroid specimen taken at surgery from 49 women with Hashimoto's thyroiditis (n=25), Graves' disease (n=15), or nodular or diffuse follicular adenomas(n=9), whose childbirth history was positive for sons. By fluorescence in situ hybridization we screened for X-chromosome and Y-chromosome-specific staining and compared the finding with human leukocyte antigen (HLA) DQ types of the mothers and, where available, their offspring. In 23 thyroids we found Y-chromosome-specific staining, which was more frequent in thyroid autoimmune disease (60% Hashimoto's thyroiditis and 40% Graves' disease) than in follicular adenomas(22.2%). There was no significant difference for HLA DQ alleles among women whose thyroids showed Y-chromosome staining and those without. However, a subgroup of all investigated microchimerism-positive mother-child pairs and women with Hashimoto's thyroiditis and Graves' disease more often had the susceptibility alleles HLA DQA1* 0501-DQB1*0201 or DQB1*0301. In conclusion, fetal microchimerism is observed in thyroids of mothers with sons, and this is found more frequently in thyroid autoimmune diseases.