One-step enzymatic synthesis of blue pigments from gemposide for fabric dyeing
One-step enzymatic synthesis of blue pigments from gemposide for fabric dyeing
复制标题
DOI：
10.1007/bf02932035
复制
发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
Shin, H. -J.
中科院分区：
文献类型：
--
作者：
Cho, Y. J.;Kim, S. Y.;Shin, H. -J.
在这项研究中，我们描述了一个一步的化学酶促反应的天然蓝色色素的生产，其中栀子提取物中的京尼平苷转化的糖苷酶京尼平。京尼平然后被允许与氨基酸反应，从而产生天然的蓝色色素。β-葡糖苷酶，最值得注意的是Isolase（β-葡聚糖酶的变体）、重组β-葡糖苷酶、纤维素酶T和淀粉酶，显示出水解京尼平苷以产生所需的色素，而α-葡糖苷酶不水解京尼平苷。在20种测试的氨基酸中，甘氨酸和酪氨酸与最高的染料产量相关。栀子苷与甘氨酸的最佳摩尔比为1。本研究生产的天然蓝色颜料用于对棉花、丝绸和羊毛进行染色。色素的颜色产量被确定为显着高于其他天然染料。此外，这些染料的色牢度性能相当好，即使在没有媒染剂的情况下。
In this study, we describe a one-step chemoenzymatic reaction for the production of natural blue pigments, in which the geniposide from Gardenia extracts is transformed by glycosidases to genipin. Genipin is then allowed to react with amino acids, thereby generating a natural blue pigment. The beta-glycosidases, most notably Isolase (a variant of beta-glucanase), recombinant beta-glucosidase, Cellulase T, and amylases, were shown to hydrolyze geniposide to produce the desired pigments, whereas the alpha-glycosidases did not. Among the 20 tested amino acids, glycine and tyrosine were associated with the highest dye production yields. The optimal molar ratio of geniposide to glycine, two reactants relevant to pigment production, was unity. The natural blue pigments produced in this study were used to dye cotton, silk, and wool. The color yields of the pigments were determined to be significantly higher than those of other natural dyes. Furthermore, the color fastness properties of these dyes were fairly good, even in the absence of mordant.