IMPROVED VISUALIZATION OF SINGLE-STRANDED AND DOUBLE-STRANDED NUCLEIC-ACIDS BY STEM
IMPROVED VISUALIZATION OF SINGLE-STRANDED AND DOUBLE-STRANDED NUCLEIC-ACIDS BY STEM
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DOI：
10.1016/0304-3991(81)90006-1
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发表时间：
1981-01-01
期刊：
影响因子：
2.2
通讯作者：
DELAIN, E
中科院分区：
文献类型：
--
作者：
MORY, C;COLLIEX, C;DELAIN, E
环形暗场STEM [扫描透射电子显微镜]图像用于显现核酸分子[噬菌体PM2双链DNA和果蝇X.病毒RNA]用醋酸双氧铀染色呈阳性。Ur簇的规则分布使得部分变性RNA分子上双链和单链片段的存在清晰可见。短至约8 nm的未配对区域可通过高效成像模式和良好适应的制备技术的这种组合来检测。
Annular dark field STEM [scanning transmission electron microscopy] images were used to visualize nucleic acid molecules [phage PM2 double-stranded DNA and Drosophila .times. virus RNA] positively stained with uranyl acetate. The regular distribution of Ur clusters makes clearly visible the existence of segments of double and single strands on partially denatured RNA molecules. Unpaired regions, as short as about 8 nm, are detectable by this combination of a highly efficient imaging mode and a well adapted preparation technique.