Olfactory subsystems in the honeybee: sensory supply and sex specificity
Olfactory subsystems in the honeybee: sensory supply and sex specificity
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DOI：
10.1007/s00441-014-1892-y
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发表时间：
2014-09-01
影响因子：
3.6
通讯作者：
Roessler, Wolfgang
中科院分区：
文献类型：
--
作者：
Kropf, Jan;Kelber, Christina;Roessler, Wolfgang
蜜蜂工蜂和雄蜂的触角在许多方面存在差异。一个显着的差异是存在的（女性）工人和他们的缺席（男性）雄蜂basiconica。我们研究了嗅感受神经元（ORN）的轴突投射模式。basiconica蜜蜂工人使用选择性顺行标记与荧光示踪剂和共聚焦显微镜分析的轴突投射在触角叶肾小球。S. basiconica相关的ORN优先投射到一个特定的肾小球集群在触角叶，即感觉输入道三（T3）集群。T3相关的肾小球先前已被证明是由内侧触角叶束（mALT）的单肾小球投射（输出）神经元支配。由于T3肾小球的数量在雄蜂中减少，我们希望确定这是否与由内侧束投射神经元支配的肾小球减少有关。我们逆行追踪mALT投射神经元在雄蜂和计数神经支配的肾小球。与工人相比，雄蜂中mALT相关肾小球的数量大幅减少。S. T3肾小球中basiconica相关的ORN以及mALT相关肾小球的减少支持了雌性（工蚁）特异性嗅觉子系统的存在，该嗅觉子系统部分受来自S.与肾小球的T3簇和mALT投射神经元相关。我们建议，这个嗅觉子系统支持并行嗅觉处理相关的工作人员特定的嗅觉任务，如编码的殖民地气味，殖民地信息素和/或气味与觅食的花卉资源。
The antennae of honeybee (Apis mellifera) workers and drones differ in various aspects. One striking difference is the presence of Sensilla basiconica in (female) workers and their absence in (male) drones. We investigate the axonal projection patterns of olfactory receptor neurons (ORNs) housed in S. basiconica in honeybee workers by using selective anterograde labeling with fluorescent tracers and confocal-microscopy analysis of axonal projections in antennal lobe glomeruli. Axons of S. basiconica-associated ORNs preferentially projected into a specific glomerular cluster in the antennal lobe, namely the sensory input-tract three (T3) cluster. T3-associated glomeruli had previously been shown to be innervated by uniglomerular projection (output) neurons of the medial antennal lobe tract (mALT). As the number of T3 glomeruli is reduced in drones, we wished to determine whether this was associated with the reduction of glomeruli innervated by medial-tract projection neurons. We retrogradely traced mALT projection neurons in drones and counted the innervated glomeruli. The number of mALT-associated glomeruli was strongly reduced in drones compared with workers. The preferential projections of S. basiconica-associated ORNs in T3 glomeruli together with the reduction of mALT-associated glomeruli support the presence of a female (worker)-specific olfactory subsystem that is partly innervated by ORNs from S. basiconica and is associated with the T3 cluster of glomeruli and mALT projection neurons. We propose that this olfactory subsystem supports parallel olfactory processing related to worker-specific olfactory tasks such as the coding of colony odors, colony pheromones and/or odorants associated with foraging on floral resources.