ATP Hydrolysis Is Critically Required for Function of CaV1.3 Channels in Cochlear Inner Hair Cells via Fueling Ca2+ Clearance
ATP Hydrolysis Is Critically Required for Function of CaV1.3 Channels in Cochlear Inner Hair Cells via Fueling Ca2+ Clearance
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DOI：
10.1523/jneurosci.4990-13.2014
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发表时间：
2014-05
期刊：
影响因子：
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通讯作者：
Simon Weiler;Stefanie Krinner;A. Wong;T. Moser;T. Pangršič
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文献类型：
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作者：
Simon Weiler;Stefanie Krinner;A. Wong;T. Moser;T. Pangršič
声音编码是由Ca2+流入介导的内毛细胞带状突触谷氨酸的释放。本文研究了ATP在这一过程中的作用，重点关注小鼠内毛细胞中Ca2+电流通过CaV1.3通道和Ca2+稳态。膜片钳记录和Ca2+成像表明，水解ATP是必不可少的，以维持突触Ca2+内毛细胞内的内流通过加油Ca2+-ATP酶，以避免增加细胞质[Ca2+]和随后的Ca2+/钙调素依赖性CaV1.3通道失活。
Sound encoding is mediated by Ca2+ influx-evoked release of glutamate at the ribbon synapse of inner hair cells. Here we studied the role of ATP in this process focusing on Ca2+ current through CaV1.3 channels and Ca2+ homeostasis in mouse inner hair cells. Patch-clamp recordings and Ca2+ imaging demonstrate that hydrolyzable ATP is essential to maintain synaptic Ca2+ influx in inner hair cells via fueling Ca2+-ATPases to avoid an increase in cytosolic [Ca2+] and subsequent Ca2+/calmodulin-dependent inactivation of CaV1.3 channels.