The transthyretin gene is expressed in Schwann cells of peripheral nerves
The transthyretin gene is expressed in Schwann cells of peripheral nerves
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DOI：
10.1016/j.brainres.2010.06.017
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发表时间：
2010-08-12
期刊：
影响因子：
2.9
通讯作者：
Sunada, Yoshihide
中科院分区：
文献类型：
--
作者：
Murakami, Tatsufumi;Ohsawa, Yutaka;Sunada, Yoshihide
甲状腺素运载蛋白（TTR）主要在肝脏和脑脉络丛中表达。大多数家族性淀粉样多发性神经病病例是由突变的TTR基因引起的。TTR沉积在周围神经系统的起源是未知的。我们研究了TTR在正常小鼠和转基因小鼠外周神经中的表达，转基因小鼠携带人类突变TTR在小鼠TTR空背景。RT-PCR检测Ttr和TTR mRNA在背根神经节和坐骨神经中均有表达。在培养的小鼠雪旺细胞和永生化小鼠雪旺细胞系IMS 32细胞中检测到Ttr mRNA。转基因小鼠培养的雪旺细胞中检测到人TTR mRNA和蛋白。我们的结论是TTR基因在周围神经的雪旺细胞中表达。(C)2010爱思唯尔有限公司版权所有。
Transthyretin (TTR) is mainly expressed in the liver and choroid plexus of the brain. The majority of familial amyloidotic polyneuropathy cases are caused by a mutant TTR gene. The origin of the TTR deposited in the peripheral nervous system is unknown. We studied the expression of TTR in the peripheral nerves of normal mice and transgenics bearing the human mutant TTR in a mouse Ttr-null background. Using RT-PCR, Ttr and TTR mRNA was observed in both dorsal root ganglia and sciatic nerves. Ttr mRNA was detected in cultured mouse Schwann cells and the immortalized mouse Schwann cell line, IMS32 cells. Human TTR mRNA and protein were detected in cultured Schwann cells derived from the transgenic mice. We conclude that the TTR gene is expressed in the Schwann cells of peripheral nerves. (C) 2010 Elsevier B.V. All rights reserved.