Choline-related metabolites influenced by feeding patterns in preterm and term infants
Choline-related metabolites influenced by feeding patterns in preterm and term infants
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DOI：
10.1080/14767058.2018.1488165
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发表时间：
2020-01-17
影响因子：
1.8
通讯作者：
Shimizu, Toshiaki
中科院分区：
文献类型：
--
作者：
Shoji, Hiromichi;Taka, Hikari;Shimizu, Toshiaki
目的：本研究旨在利用尿液代谢组分析检查足月儿和早产儿的胆碱状态。材料和方法：分别从 19 名足月儿和 20 名早产儿采集 15 天至 1 个月的样本。婴儿被分为四组：足月母乳组（TB，n = 13）、足月母乳组（TF，n = 6）、早产母乳组（PB，n = 11）和早产混合组（PM，n = 9）。使用毛细管电泳-飞行时间质谱 (CE-TOF/MS) 进行尿液代谢组分析。我们还对婴儿配方奶粉进行了代谢组分析。结果：TB 婴儿尿中胆碱代谢物（胆碱、N,N-二甲基甘氨酸、肌氨酸和甜菜碱）的排泄量显着高于 TF 婴儿 (p < .05)。胆碱、甜菜碱和肌氨酸的排泄在 PB 和 TB 婴儿之间没有显着差异。 PM 婴儿的胆碱和 N,N-二甲基甘氨酸排泄量显着高于 PB 婴儿。 PM 婴儿的胆碱代谢物排泄量也显着高于 TF 婴儿。早产儿的胆碱和甜菜碱水平显着高于本研究中使用的足月配方奶粉。结论：婴儿早期喂养方式影响胆碱代谢。代谢组分析可用于评估胆碱代谢，从而改变早产儿婴儿配方奶粉的含量。
Objective: This study was performed to examine the choline status on term and preterm infants using urinary metabolome analysis. Material and methods: Samples were collected from 19 term and 20 preterm infants between 15 days and 1 month, respectively. The infants were separated into four groups: the term-breast group (TB, n = 13), the term-formula group (TF, n = 6), the preterm-breast (PB, n = 11), and the preterm-mixed group (PM, n = 9). Urinary metabolome analysis was performed using capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF/MS). We also performed metabolome analysis of the infant formulas. Results: Urinary excretion of choline metabolites (choline, N,N-dimethylglycine, sarcosine, and betaine) was significantly higher in TB than TF infants (p < .05). Choline, betaine, and sarcosine excretion was not significantly different between the PB and TB infants. Choline and N,N-dimethylglycine excretion was significantly higher in PM than PB infants. Choline metabolites excretion was also significantly higher in PM than TF infants. Choline and betaine levels were significantly higher in the preterm than term formula used in this study. Conclusions: The type of feeding in early infancy affects choline metabolism. Metabolome analysis is useful for assessing choline metabolism to modify the contents of infant formulas also in preterm infants.