Curcumin inhibits adipogenesis induced by benzyl butyl phthalate in 3T3-L1 cells
Curcumin inhibits adipogenesis induced by benzyl butyl phthalate in 3T3-L1 cells
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DOI：
10.1016/j.taap.2017.05.036
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发表时间：
2017-08-15
影响因子：
3.8
通讯作者：
Fujimoto, Yohko
中科院分区：
文献类型：
--
作者：
Sakuma, Satoru;Sumida, Maki;Fujimoto, Yohko
邻苯二甲酸酯是一组干扰内分泌的化学物质，可能是最近全球肥胖健康危机的原因之一。通过过氧化物酶体增殖物激活受体γ(PPARγ)-CCAAT增强子结合蛋白a(C/EBPα)途径促进脂肪生成可能是邻苯二甲酸盐诱导体重增加的关键机制之一。另一方面，姜黄素已被证明在细胞和动物模型中抑制脂肪生成。本研究首次评价了姜黄素是否可以通过下调PPAR-γ-C/EBPα通路来抑制邻苯二甲酸苄基丁酯(BBP)诱导的脂肪生成。用胰岛素、地塞米松和3-异丁基-1-甲基黄嘌呤处理3T3-L1前脂肪细胞，并加入BBP，再加或不加姜黄素。单独在BBP存在下生长的细胞显示三酰甘油(TG)水平显著增加。此外，油红O染色的细胞数量和PPAR-γ、C/EBPα、脂联素和肿瘤坏死因子-α(TNF-α)的mRNA表达水平也显著增加。然而，BBP与姜黄素联合处理后，甘油三酯水平、油红O染色细胞数和四种蛋白的mRNA表达水平均显著降低。这些结果表明，姜黄素可能通过刺激脂肪细胞分化和TNFa的产生而抑制BBP诱导的体重增加和炎症。因此，姜黄素可能是一种潜在的预防邻苯二甲酸盐有害影响的药物。(C)2017 Elsevier Inc.保留所有权利。
Phthalates are a group of endocrine disrupting chemicals and may have contributed to the recent global obesity health crisis. Increased adipogenesis via the peroxisome proliferator-activated receptor gamma (PPAR gamma)-CCAAT-enhancer binding protein a (C/EBP alpha) pathway could be one critical mechanism responsible for phthalate-induced weight gain. On the other hand, curcumin has been shown to inhibit adipogenesis in cells and animal models. The present study was undertaken to evaluate, for the first time, whether curcumin could reduce adipogenesis induced by benzyl butyl phthalate (BBP) via downregulation of the PPAR gamma-C/EBP alpha pathway. 3T3-L1 preadipocytes were differentiated by treating them with insulin, dexamethasone, and 3-isobuty1-1-methylxanthine in the presence of BBP, with or without curcumin. Cells that were grown in the presence of BBP alone showed a significant increase in triacylglycerol (TG) levels. In addition, the number of Oil Red O-stained cells and the mRNA expression levels of PPAR gamma, C/EBP alpha, adiponectin, and tumor necrosis factor-alpha(TNF alpha) were significantly increased. However, treatment with BBP in combination with curcumin resulted in major reductions in TG levels, the numbers of Oil Red O-stained cells, and the mRNA expression levels of the four proteins. These results suggest that curcumin might be an inhibitor of BBP-induced weight gain and inflammation via stimulation of adipocyte differentiation and TNFa generation. Curcumin may, therefore, be a potential medication for preventing the harmful effects of phthalates. (C) 2017 Elsevier Inc All rights reserved.