Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase.
Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase.
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大肠杆菌甘氨酰胺核糖核苷酸转化酶的亚克隆、表征和亲和标记。
DOI：
10.1021/bi00458a014
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Benkovic,SJ
中科院分区：
文献类型：
--
作者：
Inglese,J;Johnson,DL;Shiau,A;Smith,JM;Benkovic,SJ
方法蛋白质测定。蛋白溶液的浓度由Pierce BCA蛋白测定法（Pierce Chemical Co.）测定。以牛血清白蛋白为标准，计算消光系数为22.1 mM'1 cm'1 （280 nm）。
Methods Protein Determination. Concentrations of protein solutions were determined by the Pierce BCA protein assay (Pierce Chemical Co.). An extinction coefficient of 22.1 mM'1 cm'1 (280 nm) was calculated by using bovine serum albumin as a standard.