Some properties and distribution of the ω-hydroxylation system of medium-chain fatty acids
Some properties and distribution of the ω-hydroxylation system of medium-chain fatty acids
复制标题
中链脂肪酸ω-羟基化体系的一些性质和分布
DOI：
10.1016/0005-2760(69)90251-3
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
Kusunosc Masamichi
中科院分区：
文献类型：
--
作者：
Ichihara Kosuke;Kusunose Emi;Kusunosc Masamichi
1. 10-羟基癸酸和癸二酸分别被大鼠肝微粒体和11000× g上清液鉴定为癸酸氧化的主要产物。2. 2.大鼠肝脏中链脂肪酸的微粒体ω-羟基化系统需要NADPH和O2。癸酸的米氏常数为2.6 μM。各种中性盐强烈地刺激了反应。CO产生了相当大的抑制作用，表明细胞色素P-450在反应中的参与。SKF 525-A和tert.-丁基异氰化物具有任何显著效果。3. 3.在各种脊椎动物（8种哺乳动物、1种鸟类、1种两栖动物和2种鱼类）的肝、肾和肺的微粒体中发现了中链脂肪酸的ω-羟基化活性。
1. 10-Hydroxydecanoate and sebacate were identified as the major products of decanoate oxidation by rat liver microsomes and 11000× g supernatant, respectively. 2. 2. The microsomal ω-hydroxylation system of medium-chain fatty acids in rat liver required NADPH and O 2. The Michaelis constant for decanoate was 2.6 μM. Various neutral salts strongly stimulated the reaction. CO produced considerable inhibition, suggesting the participation of cytochrome P-450 in the reaction. Neither SKF 525-A nor tert.-butyl isocyanide had any significant effect. 3. 3. The ω-hydroxylation activity of medium-chain fatty acids was found in the microsomes from the livers, kidneys, and lungs of various species of vertebrates (eight mammals, one bird, one amphibian and two fishes).