GENE SILENCING BY PARENTAL RNA INTERFERENCE IN THE GREEN RICE LEAFHOPPER, Nephotettix cincticeps (HEMIPTERA: CICADELLIDAE)
GENE SILENCING BY PARENTAL RNA INTERFERENCE IN THE GREEN RICE LEAFHOPPER, Nephotettix cincticeps (HEMIPTERA: CICADELLIDAE)
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DOI：
10.1002/arch.21315
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发表时间：
2016-03-01
影响因子：
2.2
通讯作者：
Hattori, Makoto
中科院分区：
文献类型：
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作者：
Matsumoto, Yukiko;Hattori, Makoto
RNA干扰（RNAi）已被广泛应用于许多非模式昆虫的基因功能研究。亲本RNAi通过向母代施用双链RNA（dsRNA）在下一代中引起基因敲低。在本研究中，我们证明了亲本RNAi介导的基因沉默是有效的决定角质层和唾液腺的基因功能的绿色稻叶蝉（GRH），黑尾叶蝉（Uhler）。将NcLac 2的dsRNA（9 ng/雌）注射到雌性亲本中，导致一龄幼虫漆酶-2基因的强烈敲除，最终导致高死亡率和身体上的侧线脱色。亲本RNAi对果蝇死亡率的影响在注射后维持了12-14天。我们还证实了亲本RNAi诱导的沉默对唾液腺中表达的基因的有效性，唾液腺中表达的基因产物从一个龄期传递到另一个龄期。亲本RNAi方法可以通过将dsRNA注射到少量亲本雌性中来对许多后代进行表型分析来检测基因功能，并且可以适用于该物种中基因功能的高效测定。(C)2015年Wiley期刊，
RNA interference (RNAi) has been widely used for investigating gene function in many nonmodel insect species. Parental RNAi causes gene knockdown in the next generation through the administration of double-strand RNA (dsRNA) to the mother generation. In this study, we demonstrate that parental RNAi mediated gene silencing is effective in determining the gene function of the cuticle and the salivary glands in green rice leafhopper (GRH), Nephotettix cincticeps (Uhler). Injection of dsRNA of NcLac2 (9 ng/female) to female parents caused a strong knockdown of laccase-2 gene of first instar nymphs, which eventually led to high mortality rates and depigmentation of side lines on the body. The effects of parental RNAi on the mortality of the nymphs were maintained through 12-14 days after the injections. We also confirmed the effectiveness of parental RNAi induced silencing on the gene expressed in the salivary gland, the gene product of which is passed from instar to instar. The parental RNAi method can be used to examine gene function by phenotyping many offspring nymphs with injection of dsRNA into a small number of parent females, and may be applicable to high-efficiency determination of gene functions in this species. (C) 2015 Wiley Periodicals,