Production of Cyclosporine A by Submerged Fermentation from a Local Isolate of Penicillium fellutanum.
Production of Cyclosporine A by Submerged Fermentation from a Local Isolate of Penicillium fellutanum.
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DOI：
10.4103/0250-474x.107082
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发表时间：
2012-07
影响因子：
0.5
通讯作者：
Irum W
中科院分区：
文献类型：
--
作者：
Anjum T;Azam A;Irum W
六株本地分离的青霉菌通过深层发酵检测了其产生环孢素A的潜力。用于药物生产的培养基由葡萄糖组成，5%；胨,1%;KH2PO4 0.5%, KCl 0.25% （w/v）。乙酸丁酯提取发酵培养基进行环孢素A分析。采用高效液相色谱法进行确证分析，并与含100 mg环孢素的诺华Sandimmun Neoral®胶囊和纯度为98.5%的外标环孢素A进行比较。只有从番石榴果实中分离得到的费芦青霉（FCBP 937）色谱峰在2.768处，与两种标准相一致。计算药量为16.18 μg/ml。
Six locally isolated strains of Penicillium were checked for their potential to produce cyclosporine A through submerged fermentation. The medium used for drug production was composed of glucose, 5%; peptone, 1%; KH2PO4, 0.5% and KCl, 0.25% (w/v). Butyl acetate was used to extract the fermentation medium for cyclosporine ′A′ analysis. The confirmation analysis was done through high performance liquid chromatography and the chromatograms obtained were compared with that of Sandimmun Neoral ®capsule (Novartis) containing 100 mg of cyclosporine and with the external standard cyclosporine A 98.5% pure. Only chromatogram of Penicillium fellutanum (FCBP 937) isolated from Guava fruit showed a peak at 2.768, which was comparable with both the standards. The amount of drug calculated was 16.18 μg/ml.