Screening and identification of resistant sources against Sclerotinia sclerotiorum causing white mold disease in common bean
Screening and identification of resistant sources against Sclerotinia sclerotiorum causing white mold disease in common bean
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DOI：
10.1002/csc2.20160
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发表时间：
2020-07-01
期刊：
影响因子：
2.3
通讯作者：
Singh, Mohar
中科院分区：
文献类型：
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作者：
Chauhan, Sonali;Katoch, Shabnam;Singh, Mohar
由白色盘菌（Sclerotinia sclerotiorum（Lib.）de Bary是菜豆（Phaseolus vulgaris L.）生产，影响植物在幼苗和成年阶段。作为小面积、资源贫乏的农民的作物，考虑到杀真菌剂的高成本，农民更喜欢使用区域特异性地方品种或抗性种质。本研究比较了大豆疫霉菌的不同接种方法。并发现改良秸秆试验接种法最为准确有效。使用这种方法，我们筛选了一组516普通豆类种质从22个国家通过国家植物遗传资源局（NBPGR;新德里，印度）对白色霉菌病原体。只有12份材料被发现抗白色霉菌，而121份材料是中等抗。在外来抗源中，EC271515、EC271475、EC271523和EC271528来自哥伦比亚地区，而EC 530910、EC 530913、EC 530916和EC 531044来自哥伦比亚地区。在来自印度的四个抗性地方品种中，IC 280353是来自查谟和克什米尔州的收集，而地方品种IC 278744、IC 278709和IC 278734来自喜马偕尔邦。综合病情进展曲线下面积、潜育期、侵染率和病斑长度等指标，EC-271475对白色霉菌的防治效果最好，其次是EC-530916。此外，统计分析还证实了表型筛选的菜豆种质的高抗性性质。一份外来材料EC271475对S.对菜豆普通花叶病毒（BCMV）NL-7 n株系也有一定的抗性，可用于菜豆育种。
White mold caused by Sclerotinia sclerotiorum (Lib.) de Bary is a devastating constraint in common bean (Phaseolus vulgaris L.) production, affecting plants at both seedling and adult stages. Being a crop of small-area, resource-poor, farmers and given the high cost of fungicides, farmers prefer the use of region-specific landraces or resistant accessions. In this study, we compared various methods of bean plant inoculation with S. sclerotiorum and found modified straw test method of inoculation as most accurate and effective. Using this method, we screened a panel of 516 common bean germplasm procured from 22 countries through the National Bureau of Plant Genetic Resources (NBPGR; New Delhi, India) against white mold pathogen. Only 12 accessions were found resistant, whereas 121 were moderately resistant to white mold. Among exotic resistant accessions, EC271515, EC271475, EC271523, and EC271528 were from the Colombian region, while the remaining four, EC530910, EC530913, EC530916, and EC531044, belonged to Columbia. Of the four resistant landraces from India, IC280353 was a collection from Jammu and Kashmir State, whereas landraces IC278744, IC278709, and IC278734 were from Himachal Pradesh. Considering the area under disease progress curve (AUDPC), incubation period, infection rate, and lesion length, EC-271475 followed by EC-530916 were concluded to be the best accessions against white mold. Further, the statistical analysis also confirms the highly resistant nature of phenotypically screened common bean accessions. One exotic accession, EC271475, possessing resistance to S. sclerotiorum was also resistant to bean common mosaic virus (BCMV) strain NL-7n and could be used in common bean breeding programs.