PROGRAMMED CELL-DEATH AND EXTRATHYMIC REDUCTION OF V-BETA-8+ CD4+ T-CELLS IN MICE TOLERANT TO STAPHYLOCOCCUS-AUREUS ENTEROTOXIN-B
PROGRAMMED CELL-DEATH AND EXTRATHYMIC REDUCTION OF V-BETA-8+ CD4+ T-CELLS IN MICE TOLERANT TO STAPHYLOCOCCUS-AUREUS ENTEROTOXIN-B
复制标题
DOI：
10.1038/349245a0
复制
发表时间：
1991-01-17
期刊：
影响因子：
64.8
通讯作者：
OCHI, A
中科院分区：
文献类型：
--
作者：
KAWABE, Y;OCHI, A
自身反应细胞的克隆缺失和功能失活已被认为是 T 细胞耐受性 1-10 胸腺内发育的潜在机制。 这些机制在更成熟的外周 T 细胞对自身或外源抗原的耐受性发展中的相对重要性尚不清楚，尽管最近的数据将外周 T 细胞耐受性的发展与克隆无反应性联系起来 11。我们现在使用 BALB/c 小鼠研究了胸腺外耐受性的诱导，这些小鼠对金黄色葡萄球菌肠毒素 B（参考文献 12）具有耐受性，这是一种在此类小鼠中与带有 T 细胞的特异相互作用的超抗原。 V-β-8抗原受体13-16。 对金黄色葡萄球菌肠毒素 B 具有耐受性的正常和无胸腺小鼠的 V-β-8.1,2+ CD4+ 外周 T 细胞数量显着减少。 这种减少伴随着与细胞死亡相关的基因组 DNA 片段化。 这些结果表明，缺失机制有助于诱导外周淋巴细胞中的 T 细胞耐受。
CLONAL deletion and functional inactivation of self-reactive cells have been invoked as mechanisms underlying intrathymic development of T-cell tolerance 1-10. The relative importance of these mechanisms in the development of tolerance of more mature, peripheral T cells either to self or to exogenous antigens is unclear, although recent data relate the development of T-cell tolerance in the periphery to clonal anergy 11. We have now investigated the induction of extrathymic tolerance using BALB/c mice that were made tolerant to Staphylococcus aureus enterotoxin B (ref. 12), a superantigen which specifically interacts in such mice with T cells bearing V-beta-8 antigen receptors 13-16. Both euthymic and athymic mice made tolerant to S. aureus enterotoxin B had a markedly reduced number of V-beta-8.1,2+ CD4+ peripheral T cells. This reduction was accompanied by genomic DNA fragmentation that is associated with cell death. These results indicate that a deletional mechanism can contribute to the induction of T-cell tolerance in peripheral lymphoid cells.