Proteomic analysis reveals that MAEL, a component of nuage, interacts with stress granule proteins in cancer cells.
Proteomic analysis reveals that MAEL, a component of nuage, interacts with stress granule proteins in cancer cells.
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DOI：
10.3892/or.2013.2836
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发表时间：
2014
期刊：
影响因子：
4.2
通讯作者：
Liqin Yuan;Yuzhong Xiao;Qiuzhi Zhou;D. Yuan;Baiping Wu;Gannong Chen;Jianlin Zhou
中科院分区：
文献类型：
--
作者：
Liqin Yuan;Yuzhong Xiao;Qiuzhi Zhou;D. Yuan;Baiping Wu;Gannong Chen;Jianlin Zhou
Maelstrom（MAEL）基因是一种癌-睾丸（或癌-生殖系）基因，其在正常条件下主要在生殖系细胞中表达，但在一系列人类癌细胞中异常表达。在生殖系细胞中，MAEL主要存在于细胞中，在皮尔纳生物发生和piRNA介导的转座子沉默中发挥重要作用。然而，MAEL在癌症中的作用尚未阐明。我们采用免疫沉淀和Nano-LC-MS/MS分析研究MAEL的相互作用组，并确定了MDA-MB-231人乳腺癌和SW 480结直肠癌细胞中14种应激颗粒（SGs）作为MAEL的潜在结合伴侣。通过抗标签免疫沉淀证实了MAEL与这些SG组分中的8种（PABPC 1、YBX 1、KHSRP、SYNCRIP、DDX 39、ELAV 1、EIF 4A 1和EIF 3F）之间的相互作用。免疫荧光分析表明，MAEL共定位与SG标记PABPC 1在SG在氧化应激。Nuages和SG分别是生殖细胞和应激体细胞的细胞质RNA颗粒，并且两者都充当小RNA介导的基因沉默的平台。因此，这表明MAEL可能参与miRNA介导的基因沉默在SGs中，因为它在nuage中。这一发现对于了解MAEL在致癌作用中的功能应该很有价值。
The Maelstrom (MAEL) gene is a cancer-testis (or cancer-germline) gene, which is predominantly expressed in germline cells under normal conditions, but is aberrantly expressed in a range of human cancer cells. In germline cells, MAEL is found predominantly in the nuage, where it plays an essential role in piRNA biogenesis and piRNA-mediated silencing of transposons. However, the role of MAEL in cancer has not been elucidated. We performed immunoprecipitation and Nano-LC-MS/MS analysis to investigate the interactome of MAEL, and identified 14 components of stress granules (SGs) as potential binding partners of MAEL in MDA-MB-231 human breast cancer and SW480 colorectal cancer cells. The interactions between MAEL and 8 of these SG components (PABPC1, YBX1, KHSRP, SYNCRIP, DDX39, ELAV1, EIF4A1 and EIF3F) were confirmed by anti-tag immunoprecipitation. Immunofluorescence analysis showed that MAEL co-localizes with the SG marker PABPC1 in SGs during oxidative stress. Nuages and SGs are the cytoplasmic RNA granules of germline cells and stressed somatic cells, respectively, and both serve as a platform for small RNA-mediated gene silencing. It is, therefore, suggested that MAEL may be involved in miRNA-mediated gene silencing in SGs, as it does in the nuage. This finding should be valuable toward understanding the function of MAEL in carcinogenesis.