EXCISION OF TRANSPOSON TN5 IS DEPENDENT ON THE INVERTED REPEATS BUT NOT ON THE TRANSPOSASE FUNCTION OF TN5
EXCISION OF TRANSPOSON TN5 IS DEPENDENT ON THE INVERTED REPEATS BUT NOT ON THE TRANSPOSASE FUNCTION OF TN5
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DOI：
10.1073/pnas.78.1.459
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
BERG, DE
中科院分区：
文献类型：
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作者：
EGNER, C;BERG, DE
通过检查 lac::Tn5 插入突变向 lac+ 的回复，研究了卡那霉素抗性决定簇 Tn5 从大肠杆菌基因组插入位点的切除。切除的频率取决于Tn5插入的位点，切除在RecA细胞中有效发生，切除不需要Tn5编码的转座功能，并且有效的切除需要Tn5的反向重复。 Tn5 的切除可能类似于自发缺失的形成，并且可能通过 DNA 合成过程中的滑动而发生。
The excision of the Kanamycin resistant determinant Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. The frequency of excision depended on the site of Tn5 insertion, excision occurred efficiently in RecA- cells, excision did not require a Tn5-encoded transposition function and efficient excision required the inverted repeats of Tn5. Excision of Tn5 may be similar to the formation of spontaneous deletions and may occur by slippage during DNA synthesis.