Transduction of endogenous envelope genes by feline leukaemia virus in vitro
Transduction of endogenous envelope genes by feline leukaemia virus in vitro
复制标题
猫白血病病毒体外转导内源包膜基因
作者：
J. Overbaugh;N. Riedel;E. Hoover;J. Mullins
猫白血病病毒（FeLV）是一种外源性逆转录病毒，可在大多数患有白血病、再生障碍性贫血、骨髓增生性疾病和致死性免疫抑制的猫中检出1，2。根据病毒分离物确定的干扰特性和体外宿主范围，将FeLV分离株分为三个亚组3，4。FeLV-A存在于所有天然分离株中4，5，并且通常具有最低限度的致病性6，7。FeLV-B与FeLV-A在约40%自然感染的分离株中以及较高比例的淋巴瘤猫中发现5。根据对禽类逆转录病毒与内源性病毒基因8的遗传重配以及AKR小鼠个体发育期间淋巴瘤病毒的起源9的基本观察，我们在此表明，用FeLV-A DNA转染猫细胞导致其与内源性FeLV相关序列重组，产生具有FeLV-B结构和宿主范围特性的病毒。因此，逆转录病毒的体外繁殖可能导致产生具有非常不同性质的变体。
Feline leukaemia viruses (FeLV) are exogenous retroviruses that can be detected in most cats with leukaemia, aplastic anaemia, myeloproliferative diseases and fatal immunosuppression1,2. FeLV isolates have been divided into three subgroups, based on the viral envelope-determined properties of interference and host range in vitro3,4. FeLV-A is present in all natural isolates4,5 and is generally minimally pathogenic6,7. FeLV-B is found with FeLV-A in isolates from ∼40% of natural infections and in a higher percentage of cats with lymphoma5. Following the fundamental observations of genetic reassortment of avian retroviruses with endogenous viral genes8 and the origination of lymphomagenic viruses during the ontogeny of AKR mice9, we show here that transfection of feline cells with FeLV-A DNA results in its recombination with endogenous FeLV-related sequences to produce viruses with the structural and host range properties of FeLV-B. Thus in vitro propagation of a retro virus may result in the generation of variants with very different properties.
DOI：
10.1073/pnas.81.22.7161
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
POTTER, H;WEIR, L;LEDER, P
通讯作者：
LEDER, P