A Monoclonal Antibody, DL10, Which Recognizes a Sugar Moiety of MHC Class I Antigens Expressed on NK Cells, NK+ T Cells, and Granulocytes in Humans
A Monoclonal Antibody, DL10, Which Recognizes a Sugar Moiety of MHC Class I Antigens Expressed on NK Cells, NK+ T Cells, and Granulocytes in Humans
复制标题
一种单克隆抗体 DL10，可识别人类 NK 细胞、NK T 细胞和粒细胞上表达的 MHC I 类抗原的糖部分
DOI：
--
复制
发表时间：
1997
影响因子：
9.1
通讯作者：
T. Abo
中科院分区：
文献类型：
--
作者：
K. Shirai;Hisami Watanabe;A. Weerasinghe;T. Sakai;H. Sekikawa;T. Abo
一个单抗，DL10，从注射了海豚淋巴细胞的小鼠中建立。除了对所有海豚淋巴细胞具有反应性外，它还能与一些人类白细胞发生反应，包括NK细胞、NK+ T细胞和粒细胞。在各种动物中检测其反应性时，牛、羊和马的白细胞均为DL10+。小鼠、大鼠和犬的白细胞为DL10−。虽然DL10+在人体内的反应性与CD56和CD57抗原相似，但它所识别的实际分子是不同的。因此，用糖肽酶处理细胞或用tunicamycin培养细胞后，DL10的反应性全部消失。此外，免疫沉淀法显示DL10间接识别人和动物MHC I类抗原重链（45kD）。结合DL10分子n端氨基酸序列分析和反应细胞HLA分型数据，DUO识别了一些单态MHC抗原和多态MHC抗原（如HLA- b60和-B61）的糖片段。如果供体是HLA-B60−和-B61−(>在日本为80%，>在美国为95%)，DL10似乎是一种非常有用的检测泛nk + T细胞的试剂。
One mAb, DL10, was established from mice injected with dolphin lymphocytes. In addition to its reactivity against all dolphin lymphocytes, it reacted with some human leukocytes, including NK cells, NK+ T cells, and granulocytes. When its reactivity was examined in various animals, bovine, ovine, and equine leukocytes were DL10+. Murine, rat, and canine leukocytes were DL10−. Although the reactivity of DL10+ was similar to those of CD56 and CD57 antigens in humans, the actual molecules it recognized were different. Thus, all reactivity of DL10 disappeared after treatment of cells with glycopeptidase or after culture of cells with tunicamycin. Furthermore, the immunoprecipitation method revealed that DL10 indirectly recognized the heavy chain (45kD) of MHC class I antigen in humans and animals. Considering data from analysis of the N-terminal amino acid sequence of the DL10 molecule and the HLA typing of reactive cells, DUO recognized a sugar moiety of some monomorphic MHC antigens and polymorphic MHC antigens such as HLA-B60 and -B61. If the donors are HLA-B60− and -B61− (>80% in Japan and >95% in the United States), DL10 would appear to be a very useful agent for the detection of pan-NK+ T cells.
由活化的 CD4 或 CD8 T 细胞产生小淋巴细胞刺激 1a 抗原。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Arase-Fukushi,N;Arase,H;Ogasawara,K;Good,RA;Onoé,K
通讯作者：
Onoé,K
鼠肝 T 细胞的 V beta 库。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Seki,S;Kono,DH;Balderas,RS;Theofilopoulos,AN
通讯作者：
Theofilopoulos,AN
DOI：
10.1073/pnas.90.4.1359
发表时间：
1993-02-15
影响因子：
11.1
作者：
NEEDHAM, LK;SCHNAAR, RL
通讯作者：
SCHNAAR, RL