Valorization of Citrus limon residues for the recovery of antioxidants: Evaluation and optimization of microwave and ultrasound application to solvent extraction
Valorization of Citrus limon residues for the recovery of antioxidants: Evaluation and optimization of microwave and ultrasound application to solvent extraction
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DOI：
10.1016/j.indcrop.2013.07.013
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发表时间：
2013-10-01
影响因子：
5.9
通讯作者：
Madani, Khodir
中科院分区：
文献类型：
--
作者：
Dahmoune, Farid;Boulekbache, Lila;Madani, Khodir
采用响应面法（RSM）优化超声辅助提取（UAE）和微波辅助提取（MAE），并比较柑橘利蒙皮中总酚类化合物（TPC表示为没食子酸当量（GAE））的回收率。微波辅助提取的最佳工艺条件为：提取溶剂为48%乙醇，液固比为28：1 mL/g，辐射时间为123 s，辐射功率为400 W。超声波辅助提取的最佳工艺条件为：乙醇浓度63.93%，液固比40 mL/g，提取时间15.05 min，提取幅度77.79%。在MAE和UAE的优化条件下，TPC的最大预测回收率分别为15.74和15.08 mg GAE/g，与实验值15.78 +/- 0.8和15.22 +/- 0.88 mg GAE/g接近，表明所采用的模型适用，RSM在优化提取条件方面取得了成功。通过DPPH和还原力测试确定的抗氧化活性证实了MAE用于制备富含抗氧化剂的植物提取物的适用性。(C)2013爱思唯尔有限公司版权所有。
Ultrasound assisted extraction (UAE) and microwave-assisted extraction (MAE) were optimized (by response surface methodology, RSM) and compared for the recovery of total phenolic compounds (TPC expressed as gallic acid equivalents (GAE)) from Citrus limon peels. The optimized result for MAE was 48% ethanol as extraction solvent, 28:1 mL/g of solvent: solid ratio, 123 s and 400 W for irradiation time and power. The optimized result for UAE was 63.93% ethanol as extraction solvent, 40 mL/g of liquid/solid ratio, 15.05 min of holding time and 77.79% for amplitude. Maximum predicted TPC recoveries under the optimized conditions for MAE and UAE were 15.74 and 15.08 mg GAE/g respectively, which were close to the experimental values of 15.78 +/- 0.8 and 15.22 +/- 0.88 mg GAE/g, indicating suitability of the employed model and the success of RSM in optimizing the extraction conditions. The antioxidant activity determined by the DPPH and reducing power tests confirmed the suitability of MAE for the preparation of antioxidant-rich plant extracts. (C) 2013 Elsevier B.V. All rights reserved.