Assembly of functionally active Drosophila origin recognition complex from recombinant proteins
Assembly of functionally active Drosophila origin recognition complex from recombinant proteins
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DOI：
10.1101/gad.13.10.1289
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发表时间：
1999-05-15
影响因子：
10.5
通讯作者：
Botchan, M
中科院分区：
文献类型：
--
作者：
Chesnokov, I;Gossen, M;Botchan, M
In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of origin usage in Drosophila is not regulated solely by a large family of different ORC proteins. The six-subunit ORC can be reconstituted with recombinant proteins into a complex that restores DNA replication in ORC-depleted Drosophila or Xenopus egg extracts.
