Dietary supplementation of a natural isomer mixture of beta-carotene inhibits oxidation of LDL derived from patients with diabetes mellitus
Dietary supplementation of a natural isomer mixture of beta-carotene inhibits oxidation of LDL derived from patients with diabetes mellitus
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DOI：
10.1159/000012821
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发表时间：
2000-03-01
影响因子：
3.9
通讯作者：
Aviram, M
中科院分区：
文献类型：
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作者：
Levy, Y;Zaltsberg, H;Aviram, M
背景：加速的动脉粥样硬化在糖尿病患者中很常见，这可能与脂质过氧化作用增加有关。因此，我们比较了来自糖尿病患者和血糖正常对照的LDL氧化，并随访了膳食β-胡萝卜素补充剂对LDL氧化的影响。研究方法：20例长期非胰岛素依赖型糖尿病患者与年龄和性别匹配的对照组进行了比较研究。对患者补充杜氏盐藻来源的β-胡萝卜素，每日60 mg，持续3周。LDL氧化分析通过测量丙二醛（MDA），脂质过氧化物（PD），共轭二烯（CD）的生成响应CuSO 4诱导的氧化。LDL脂质成分和LDL相关的维生素A，E和类胡萝卜素也进行了测量。结果如下：患者中LDL对CuSO 4氧化的敏感性增加了40%，LDL氧化开始所需的滞后时间缩短了35%，即患者为56 +/- 6 min，对照组为85 +/- 9 min（p < 0.01）。患者显示胆固醇/磷脂和多不饱和/饱和比率增加，以及LDL相关维生素含量降低。补充β-胡萝卜素后，血浆和LDL全反式β-胡萝卜素显著升高[从0.296 +/- 0.020至0.968 +/- 0.133 μ g/mg LDL蛋白（p < 0.01）]由于LDL对氧化的敏感性显著降低，如延迟时间增加至115 +/-10分钟（p < 0.01）和MDA和PD生成分别减少（25%和40%）（p < 0.01）所示。结论：来自糖尿病患者的LDL对氧化的敏感性增加与LDL脂质组成和抗氧化剂含量异常相关。天然β-胡萝卜素膳食补充剂使增强的LDL氧化正常化，因此可能对延缓这些患者动脉粥样硬化的加速发展具有重要意义。版权所有（C）2000 S. Karger AG，巴塞尔。
Background: Accelerated atherosclerosis is common in patients with diabetes mellitus which may be linked to increased lipid peroxidation. Therefore, we compared the oxidation of LDL derived from patients with diabetes to normoglycemic controls and followed-up the effect of dietary beta-carotene supplementation on LDL oxidation. Methods: Twenty patients with long-standing non-insulin-dependent diabetes mellitus were studied in comparison with age- and sex-matched control subjects. Dunaliella bardawil-derived beta-carotene was supplemented to the patients for 3 weeks, 60 mg daily dose. LDL oxidation was analyzed by measuring malondialdehyde (MDA), lipid peroxides (PD), and conjugated dienes (CD) generation in response to CuSO4-induced oxidation. LDL lipid composition and the LDL associated vitamins A, E and carotenoids were also measured. Results: LDL susceptibility to oxidation by CuSO4 was increased in the patients by 40% with a 35% shorter lag time required for the initiation of LDL oxidation, i.e. 56 +/- 6 min in patients vs. 85 +/- 9 min in controls (p < 0.01). Patients showed increased cholesterol/phospholipid and polyunsaturated/saturated ratios, as well as reduced content of LDL associated vitamins. Upon beta-carotene supplementation, there was a significant elevation in plasma and in LDL all-trans beta-carotene [from 0.296 +/- 0.020 to 0.968 +/- 0.133 mu g/mg LDL protein (p < 0.01)] paralleled by a significant reduction in LDL susceptibility to oxidation, as exhibited by increased lag time up to 115 +/- 10 min (p < 0.01) and reduction in MDA and PD generation (by 25 and 40%), respectively (p < 0.01). Conclusions: Increased susceptibility to oxidation of LDL derived from patients with diabetes mellitus is associated with abnormal LDL lipid composition and antioxidant content. Natural beta-carotene dietary supplementation normalizes the enhanced LDL oxidation and consequently may be of importance in delaying accelerated development of atherosclerosis in these patients. Copyright (C) 2000 S. Karger AG, Basel.