Isolation and characterization of human pituitary chorionic gonadotropin
Isolation and characterization of human pituitary chorionic gonadotropin
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DOI：
10.1210/en.137.4.1402
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发表时间：
1996-04-01
期刊：
影响因子：
4.8
通讯作者：
Hartree, AS
中科院分区：
文献类型：
--
作者：
Birken, S;Maydelman, Y;Hartree, AS
人体CG （hCG）是一种与维持妊娠有关的激素。虽然有三种相关的糖蛋白激素LH、kFSH和hTSH由垂体分泌，但hCG是该糖蛋白激素家族中唯一一种由灵长类动物胎盘产生以维持黄体类固醇激素分泌的糖蛋白激素。虽然hCG不被认为是一种垂体激素，但20年来在健康未怀孕个体的垂体组织、血液和尿液中报道了hCG样免疫反应物质，但从未分离过。我们现在报道从丙酮保存的人垂体腺中纯化和表征垂体hCG。凝胶渗透层析后，将含有hCG分子的组分集合起来，利用hCG β cooh末端区的抗体进行免疫亲和层析纯化。氨基酸分析、氨基末端序列分析以及质谱研究对垂体hCG和从孕妇尿液中纯化的hCG都给出了相似的结果。硫酸根和唾液酸含量分析表明垂体hCG同时含有硫酸根和唾液酸。垂体hCG的体外生物活性表明，在cAMP测定中，它的活性是孕妇尿液中纯化的hCG的50%。
Human CG (hCG) is the hormone associated with the maintenance of pregnancy. Although three related glycoprotein hormones, LH, kFSH, and hTSH, are secreted by the pituitary, hCG is the only one of this family of glycoprotein hormones that is produced by the placenta in primates to maintain the steroid hormone secretions of the corpus luteum. Although hCG is not considered to be a pituitary hormone, hCG-like immunoreactive materials have been reported in pituitary tissue, blood, and urine from healthy nonpregnant individuals for 2 decades, but it was never isolated. We now report the purification and characterization of pituitary hCG from acetone-preserved human pituitary glands. After gel permeation chromatography, the fractions containing hCG molecules were pooled and purified by immunoaffinity chromatography using antibodies to the COOH-terminal region of hCG beta. Amino acid analyses, amino-terminal sequence analyses, as well as mass spectrometric studies gave similar results for both pituitary hCG and hCG purified from the urine of pregnant women. Analyses for sulfate and sialic acid contents demonstrated that pituitary hCG contained both sulfate and sialic acid. In vitro biological activity of pituitary hCG indicated that it was 50% as active as hCG purified from the urine of pregnant women in cAMP assays.