Production of β2‐Microglobulin by Normal and Malignant Human Cell Lines and Peripheral Lymphocytes
Production of β2‐Microglobulin by Normal and Malignant Human Cell Lines and Peripheral Lymphocytes
复制标题
正常和恶性人类细胞系和外周淋巴细胞产生 β2-微球蛋白
作者：
K. Nilsson;P. Evrin;K. Welsh
/JS-微球蛋白是一种低分子量蛋白质，最初由Berggard&Beam(1968年)从人尿中分离出来，分子量为11.800。/？2-/I后来在人的血清、脑脊液、唾液和初乳中被发现(Berggard&Bearn 1968，Evrin et al.1971年)。对于正常人来说，血清水平的变化范围很小(Evrin&Wibell 1972)。肾脏疾病患者的血清中经常记录到高水平(Bemier等人。1968年，Peterson et al.1969年，Wibell等人。1973年)，并且在恶性疾病患者中不规律(Evrin&Wibell 1973，Kithier等人)。1974年)。对一级结构的研究表明，p2-u与人免疫球蛋白的恒定结构域具有高度的同源性(Peterson等人。1972年，Smithies&Poulik 1972)。这一发现和^2-/^具有链内二硫键环的事实表明，/？2-/(是一个具有与免疫球蛋白的C-H3结构域相似的效应功能的游离免疫球蛋白结构域(Peterson et al.1972年)。因此，早期关于人类细胞产生^2-^的工作主要集中在淋巴细胞上。Peterson et al.(1972)和Evrin&Pertoft(1973)的研究表明，细胞表面存在^2-fi.
/Js-microglobulin {[^T/I) is a low molecular weight protein (MW 11.800) originally isolated by Berggard & Beam (1968) from human urine. /?2-/i was later found in human serum, cerebrospinal fluid, saliva and colostrum (Berggard & Bearn 1968, Evrin et al. 1971). The serum levels have been shown to vary within a narrow range for normal individuals (Evrin & Wibell 1972). High levels are recorded regularly in sera from patients with renal diseases (Bemier et al. 1968, Peterson et al. 1969, Wibell et al. 1973) and irregularly in patients with malignant diseases (Evrin & Wibell 1973, Kithier et al. 1974). Studies of the primary structure have shown a high degree of homology of p2-u with the constant domains of human IgG (Peterson et al. 1972, Smithies & Poulik 1972). This finding and the fact that ^2-/^ has an intrachain disulphide loop suggested that /?2-/( was a free immunoglobulin domain with effector function similar to that of the C H 3 domain of IgG (Peterson et al. 1972). Consequently early work on the production of ^2-^ by human cells was concentrated on lymphocytes. Peterson et al. (1972) and Evrin & Pertoft (1973) showed that ^2-fiwas present at the cell surface of