The Gpmk1 MAP kinase of Fusarium graminearum regulates the induction of specific secreted enzymes
The Gpmk1 MAP kinase of Fusarium graminearum regulates the induction of specific secreted enzymes
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DOI：
10.1007/s00294-004-0547-z
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发表时间：
2005-01-01
期刊：
影响因子：
2.5
通讯作者：
Schäfer, W
中科院分区：
文献类型：
--
作者：
Jenczmionka, NJ;Schäfer, W
最近，我们描述了禾谷镰刀菌的Gpmk1 MAP激酶干扰突变体，它们在体外完全存活，但完全失去了侵染小麦的能力。由于细胞壁降解酶被认为参与了禾谷镰刀菌的感染过程，因此对这些MAP激酶破坏突变体在体外产生细胞壁降解酶的能力进行了分析，并与野生型菌株进行了比较。Gpmk1基因的中断对果胶分解酶或淀粉分解酶的产生没有影响。然而，Gpmk1调节细胞外内葡聚糖酶、木聚糖酶和蛋白水解酶的早期诱导。此外，MAP激酶负责整个分泌性脂解活性的诱导。由于Gpmk1 MAP激酶的破坏导致了一种非致病表型，这些结果表明禾谷镰刀菌的感染过程依赖于细胞壁降解酶的分泌，特别是在感染的早期阶段。最后，本工作首次详细分析了禾谷镰刀菌Gpmk1突变体的致病表型。
Recently, we described Gpmk1 MAP kinase-disruption mutants of Fusarium graminearum that were fully viable in vitro, but had completely lost their ability to infect wheat. As cell wall-degrading enzymes are postulated to participate in the infection process of F. graminearum, these MAP kinase-disruption mutants were analysed for their ability to produce cell wall-degrading enzymes in vitro and compared with the wild-type strain. The gpmk1 disruption had no effect on the production of pectinolytic or amylolytic enzymes. However, Gpmk1 regulates the early induction of extracellular endoglucanase, xylanolytic, and proteolytic activities. Furthermore, the MAP kinase was responsible for the overall induction of secreted lipolytic activities. Since the disruption of the Gpmk1 MAP kinase leads to an apathogenic phenotype, these results suggest that the infection process of F. graminearum depends on the secretion of cell wall-degrading enzymes, particularly during the early infection stage. Finally, this work provides the first detailed analysis of the apathogenic phenotype of the F. graminearum Gpmk1 mutants.