NATURAL KILLER CELLS IN MOUSE .2. CYTOTOXIC CELLS WITH SPECIFICITY FOR MOUSE MOLONEY LEUKEMIA-CELLS - CHARACTERISTICS OF KILLER CELL
NATURAL KILLER CELLS IN MOUSE .2. CYTOTOXIC CELLS WITH SPECIFICITY FOR MOUSE MOLONEY LEUKEMIA-CELLS - CHARACTERISTICS OF KILLER CELL
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DOI：
10.1002/eji.1830050209
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发表时间：
1975-01-01
影响因子：
5.4
通讯作者：
WIGZELL, H
中科院分区：
文献类型：
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作者：
KIESSLING, R;KLEIN, E;WIGZELL, H
正常小鼠含有对体外培养的小鼠莫洛尼白血病细胞具有特异性的细胞溶解细胞。这种杀伤细胞最常见于脾脏；淋巴结和骨髓含有较少，胸腺几乎没有杀伤活性。在一到三个月大的时候，活动达到高峰。来自基因胸腺小鼠的脾脏细胞与来自同一品系的正常小鼠的脾脏细胞一样具有活性杀伤细胞。用抗- theta血清加补体处理，然后通过抗- Ig柱过滤去除附着和表面Ig阳性细胞，将留下正常小鼠原始脾脏细胞群的1 - 5%。这些细胞具有小淋巴细胞的形态，可能含有脾脏对Moloney白血病细胞的全部原始杀伤活性。这种杀手富集细胞缺乏T淋巴细胞和B淋巴细胞，在抗体诱导、细胞介导的裂解中对抗体包被的鸡红细胞不起作用。由此可见，正常小鼠脾细胞对Moloney白血病细胞的自发选择性细胞毒活性是由性质不明的小淋巴细胞发挥的。
Normal mice contain cytolytic cells with specificity forin vitrogrown mouse Moloney leukemia cells. Such killer cells are most frequent in the spleens; lymph node and bone marrow contain less and thymus virtually no killer activity. Peak activity is found around one to three months of age. Spleen cells from genetically athymic mice are as active killer cells as those from normal mice of the same strain. Treatment with anti‐theta serum plus complement followed by removal of adherent and surface Ig positive cells by filtration through anti‐Ig columns will leave between 1–5 % of the original spleen cell population from a normal mouse. These cells have the morphology of small lymphocytes and perhaps contain all of the total original killer activity of the spleen against the Moloney leukemia cells. Such killer enriched cells are devoid of T and B lymphocytes and largely fail to function in antibody induced, cell‐mediated lysis against antibody‐coated chicken erythrocytes. It is concluded that the spontaneous selective cytotoxic activity of normal mouse spleen cells against Moloney leukemia cells is exerted by small lymphocytes of yet undefined nature.