Intracerebroventricular administration of growth hormone releasing peptide-6 (GHRP-6) inhibits food intake, but not food retention of crop and stomach in neonatal chicks
Intracerebroventricular administration of growth hormone releasing peptide-6 (GHRP-6) inhibits food intake, but not food retention of crop and stomach in neonatal chicks
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DOI：
10.2141/jpsa.43.35
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发表时间：
2006-01-01
影响因子：
1.5
通讯作者：
Bungo, Takashi
中科院分区：
文献类型：
--
作者：
Khan, Md. Sakirul Islam;Dodo, Koh-Ichi;Bungo, Takashi
生长激素分泌物(GHS)，包括Ghrelin，已知可以刺激哺乳动物的摄食行为。相反，侧脑室(Icv)注射大鼠或鸡的Ghrelin会强烈地抑制雏鸡的食物摄入量。这种差异促使我们比较了生长激素释放肽-6(GHRP-6)和生长激素释放素(Ghrelin)对雏鸡饲养行为的影响。体内实验还检测了GHRP-6的胃动力刺激作用，测量了食物在消化道的滞留量。侧脑室注射GHRP-6(0.38nmo1-1.5nmo1)可剂量依赖性地抑制新生雏鸡在自由和禁食条件下的摄食量。GHS受体拮抗剂D-Lys(3)-GHRP-6可剂量依赖性地减弱GHRP-6的厌食作用。中枢注射GHS激动剂和/或拮抗剂不影响作物、腺胃和肌胃的食物滞留。这些结果表明，中心注射合成的GHS，GHRP-6抑制蛋鸡的摄食行为，剂量为Ghrelin，而不影响胃肠运动。
Growth hormone secretagogues (GHS), including ghrelin, are known to stimulate feeding behavior in mammals. In contrast, intracerebroventricular (ICV) injection of rat or chicken ghrelin, strongly inhibits food intake in chicks. The difference prompted us to compare the effect of growth hormone releasing peptide-6 (GHRP-6), one of synthetic GHSs on feeding behavior of chicks with that of ghrelin. Gastric motility stimulating action of GHRP-6 was also examined in vivo measuring retention of food in digestive tracts. ICV injection of GHRP-6 (0.38 nmol-1.5 nmol) dose-dependently inhibited food intake of neonatal chicks under both ad libitum and fasting conditions. The anorexic effect of GHRP-6 was attenuated by D-Lys(3)-GHRP-6 (GHS receptor antagonist) in a dose dependent manner. Central injection of GHS agonist and/or antagonist did not affect food retention in crop, proventriculus and gizzard. These results suggest that central injection of synthetic GHS, GHRP-6 inhibits feeding behavior as dose ghrelin without affecting gastrointestinal motility in layer chicks.