Detection of rhinovirus in induced sputum at exacerbation of chronic obstructive pulmonary disease
Detection of rhinovirus in induced sputum at exacerbation of chronic obstructive pulmonary disease
复制标题
DOI：
10.1034/j.1399-3003.2000.16d19.x
复制
发表时间：
2000-10-01
影响因子：
24.3
通讯作者：
Wedzicha, JA
中科院分区：
文献类型：
--
作者：
Seemungal, TAR;Harper-Owen, R;Wedzicha, JA
普通感冒与慢性阻塞性肺疾病（COPD）的加重有关，然而，普通感冒病毒（人鼻病毒）在COPD加重时症状和下气道炎症的产生中的作用尚不清楚，在基线时观察到33例中度至重度COPD患者，当时注意到前一年的胸部感染数量，并在COPD加重时急性发作。在急性发作后48小时内和基线时，采集鼻抽吸物和诱导痰进行鼻病毒逆转录酶聚合酶链反应（RT-PCR）分析和细胞因子水平测定。在日记卡上记录症状，并测量一秒用力呼气量（FEV 1）和用力肺活量（FVC）。
Common colds are associated with exacerbations of chronic obstructive pulmonary disease (COPD), However, the role of the common cold virus (human rhinovirus) in the production of symptoms and lower airway inflammation at COPD exacerbation is unknown,Thirty three patients with moderate-to-severe COPD were seen at baseline, when the number of chest infections in the previous year was noted, and acutely at COPD exacerbation. Within 48 h after the onset of the exacerbation and at baseline, nasal aspirates and induced sputum were taken for rhinovirus reverse transcriptase polymerase chain reaction (RT-PCR) analysis and determination of cytokine levels. Symptoms, recorded on diary cards, were noted and forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) measured.At exacerbation, mean FEV1 and FVC fell significantly from baseline (p