HIGH DENSITY FERMENTATION OF RECOMBINANT E.coli FOR PRODUCTION OF HUMAN-LIKE COLLAGEN
HIGH DENSITY FERMENTATION OF RECOMBINANT E.coli FOR PRODUCTION OF HUMAN-LIKE COLLAGEN
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发表时间：
2002
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通讯作者：
Jirong Song
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作者：
Jirong Song
研究了重组大肠杆菌发酵生产类胶原生物聚合物过程中的几个工艺参数，观察了溶解氧和葡萄糖浓度对发酵的影响，当溶解氧控制在50%、葡萄糖在1%左右时，OD 600 值可以达到98。获得了类人胶原蛋白的产率29.4%。
Several technical parameters were studied during the fermentation of recombinant E.coli for the production of collagen-like biopolymer.The effects of dissolved oxygen as well as glucose concentration on fermentation were observed.The OD 600 value could reach 98 when dissolved oxygen was controlled at 50% and glucose around 1%.The production of human-like collagen with a yield of 29.4% was obtained.