IL-33 Expression in the Cerebral Cortex Following Experimental Subarachnoid Hemorrhage in Rats
IL-33 Expression in the Cerebral Cortex Following Experimental Subarachnoid Hemorrhage in Rats
复制标题
大鼠实验性蛛网膜下腔出血后大脑皮层中 IL-33 的表达。
DOI：
10.1007/s10571-014-0143-9
复制
发表时间：
2015-05-01
影响因子：
4
通讯作者：
Hang, Chun-hua
中科院分区：
文献类型：
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作者：
Huang, Li-tian;Li, Hua;Hang, Chun-hua
蛛网膜下腔出血（SAH）是一种广泛存在的破坏性疾病，其中炎症和凋亡途径导致预后不良。白细胞介素-33（IL-33）通过与跨膜ST 2受体结合，在炎症和凋亡途径中起着至关重要的作用。本研究旨在探讨IL-33在SAH后大脑皮质的表达和细胞定位，以阐明IL-33在SAH后的作用。将SD大鼠随机分为假手术组和SAH组，分别于术后2、6、12 h和1、2、3、5 d进行评估，SAH动物接受视交叉前池SAH。IL-33的表达通过蛋白质印迹分析、实时PCR、免疫组织化学和免疫荧光来测量。还评估了肿瘤坏死因子（TNF）-α和IL-1 β的mRNA水平。与假手术组相比，SAH组IL-33、IL-1 β和TNF-α的表达显著升高; SAH后IL-33主要定位于神经元和星形胶质细胞，而不是小胶质细胞。此外，在IL-33和IL-1 β表达之间观察到显著的正相关。提示IL-33可能在SAH后的炎症反应中起重要作用。
Subarachnoid hemorrhage (SAH) is a pervasive and devastating condition in which inflammatory and apoptotic pathways contribute to poor outcome. Interleukin-33 (IL-33) plays a crucial role in the inflammatory and apoptotic pathways through binding of the transmembrane ST2 receptor. This study investigated the expression and cellular localization of IL-33 in the cerebral cortex after SAH in order to clarify the role of IL-33 after SAH. Sprague-Dawley rats were randomly divided into sham and SAH groups and evaluated 2, 6, and 12 h and 1, 2, 3, and 5 days after the surgery, with SAH animals subjected to prechiasmatic cistern SAH. IL-33 expression was measured by western blot analysis, real-time PCR, immunohistochemistry, and immunofluorescence. The mRNA levels of tumor necrosis factor (TNF)-alpha and IL-1 beta were also assessed. The expression of IL-33, IL-1 beta, and TNF-alpha was markedly elevated in the SAH as compared to the sham group; IL-33 was mainly localized in neurons and astrocytes and not microglia after SAH. Moreover, a significant positive association was observed between IL-33 and IL-1 beta expression. These findings indicate that IL-33 might play an important role in the inflammatory response following SAH.