Effect of oxidative stress on respiratory epithelium from children with Down syndrome
Effect of oxidative stress on respiratory epithelium from children with Down syndrome
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DOI：
10.1183/09031936.00122812
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发表时间：
2013-10-01
影响因子：
24.3
通讯作者：
van Woensel, Job B. M.
中科院分区：
文献类型：
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作者：
Bruijn, Martijn;Lutter, Rene;van Woensel, Job B. M.
患有唐氏综合症的儿童患急性呼吸窘迫综合症的风险很高。在唐氏综合征中，炎症和细胞凋亡的调节都是异常的，这在急性呼吸窘迫综合征的病理生理学中很重要。这与自由基清除剂的不平衡有关。我们研究了自由基清除剂的表达和氧化应激对唐氏综合征儿童呼吸道上皮细胞凋亡和炎症的影响，并与对照组进行了比较。我们培养了唐氏综合征儿童（n=12）和对照组（n=17）的鼻上皮细胞。首先，我们发现自由基清除剂CuZn-超氧化物歧化酶的表达比对照组高28与对照组相比，唐氏综合征儿童的过氧化氢酶降低36%（p=0.04），谷胱甘肽过氧化物酶降低73%（p=0.004）。我们没有发现显着差异，细胞凋亡，唐氏综合征和对照组之间暴露于氧化应激。我们还发现，在唐氏综合征和对照组之间，暴露于氧化应激后，原代鼻上皮细胞上清液中白细胞介素（IL）-1 β、IL-6、IL-8、血管内皮生长因子和粒细胞集落刺激因子的水平没有显著差异。我们发现唐氏综合征儿童呼吸道上皮细胞中自由基清除剂的失衡，但这并不导致暴露于氧化应激时细胞凋亡或炎症水平的增加。
Children with Down syndrome are at high risk for acute respiratory distress syndrome. In Down syndrome, both regulation of inflammation and apoptosis, important in acute respiratory distress syndrome pathophysiology, are abnormal. This has been linked to an imbalance in free radical scavengers. We investigated the expression of free radical scavengers and the effect of oxidative stress in terms of apoptosis and inflammation in respiratory epithelium from children with Down syndrome compared with control subjects.We cultured primary nasal epithelial cells from Down syndrome children (n=12) and controls (n=17) and exposed them to oxidative stress by supplementing superoxide.First we showed that the expression of the free radical scavengers CuZn-superoxide dismutase was 28% higher (p=0.06), catalase was 36% lower (p=0.04) and glutathione peroxidase was 73% lower (p=0.004) in Down syndrome children compared with controls. We found no significant difference in apoptosis, between Down syndrome and control subjects after exposure to oxidative stress. We also found no significant difference in levels of interleukin (IL)-1 beta, IL-6, IL-8, vascular endothelial growth factor and granulocyte colony-stimulating factor in primary nasal epithelial cell supernatant after exposure to oxidative stress between Down syndrome and control subjects.We found an imbalance in free radical scavengers in respiratory epithelial cells from children with Down syndrome, but this did not result in increased levels of either apoptosis or inflammation upon exposure to oxidative stress.