PURINERGIC INNERVATION OF GUINEA-PIG URINARY-BLADDER
PURINERGIC INNERVATION OF GUINEA-PIG URINARY-BLADDER
复制标题
DOI：
10.1111/j.1476-5381.1978.tb07782.x
复制
发表时间：
1978-01-01
影响因子：
7.3
通讯作者：
KASAKOV, L
中科院分区：
文献类型：
--
作者：
BURNSTOCK, G;COCKS, T;KASAKOV, L
1研究了豚鼠膀胱腺苷5‘-三磷酸(ATP)作为神经递质的若干标准。此外，对三磷酸腺苷的快速反应对非肾上腺素、非胆碱能神经刺激的反应的影响也被重新研究。2奎纳克林荧光组织化学显示膀胱内有大量神经纤维、神经节细胞和神经束，虹膜和输精管均未见神经纤维、神经节细胞和神经束，以肾上腺素能和胆碱能神经为主。其分布和形态与儿茶酚胺荧光和胆碱酯酶组织化学不同，不受化学交感神经切除的影响。3刺激肌壁内兴奋性神经时，在阿托品和金刚乙胺存在下，膀胱释放的三磷酸腺苷增加到刺激前的3~12倍。河豚毒素可阻断由壁内神经刺激引起的收缩反应和ATP释放的增加。在河豚毒素神经麻痹后，由于直接肌肉刺激导致的收缩过程中，ATP的释放没有增加。4交感神经切除加6-羟基多巴胺不影响壁内神经刺激引起的ATP释放。5三磷酸腺苷的释放依赖于培养液中钙离子的浓度。6非肾上腺素能、非胆碱能壁内神经刺激的收缩反应可被三磷酸腺苷(ATP)紧密地模拟，但不能被乙酰胆碱或组胺所模拟。7腺苷和潘生丁可降低对ATP和非胆碱能神经刺激的收缩。8 2-2‘-吡啶醇不是刺激豚鼠膀胱壁内神经或三磷酸腺苷的特异性阻断剂。2-取代咪唑类化合物引发自发活动，因此不可能评估它们可能具有的任何阻断作用。9前列腺素(E_1、E_2和F_2α)表现为微弱、缓慢的收缩和自发活动的增加。前列腺素对ATP和非肾上腺素、非胆碱能神经刺激的反应均显著增强。10在吲哚美辛存在下，对非肾上腺素、非胆碱能神经刺激的反应在对三磷酸腺苷脱敏后几乎消失。图片
1 A number of criteria for considering adenosine 5′-triphosphate (ATP) as a neurotransmitter in the guinea-pig urinary bladder have been examined. In addition, the effect of tachyphylaxis to ATP on the response to non-adrenergic, non-cholinergic nerve stimulation has been re-examined. 2 Quinacrine fluorescence histochemistry revealed a population of nerve fibres, ganglion cells, and nerve bundles in the bladder which were not seen in either the iris or vas deferens, where adrenergic and cholinergic nerves predominate. The distribution and morphology of the quinacrine-positive nerves in the bladder were different from those observed with catecholamine fluorescence and cholinesterase histochemistry, and were unaffected by chemical sympathectomy. 3 Release of ATP from the bladder during stimulation of intramural excitatory nerves, in the presence of atropine and guanethidine increased to 3-12 times prestimulation levels. Tetrodotoxin abolished both the contractile response and the increase in ATP release resulting from intramural nerve stimulation. There was no increase in ATP release during contraction resulting from direct muscle stimulation following nerve paralysis with tetrodotoxin. 4 Sympathectomy with 6-hydroxydopamine did not affect release of ATP in response to intramural nerve stimulation. 5 Release of ATP was dependent on the concentration of calcium ion in the medium. 6 Contractions in response to non-adrenergic, non-cholinergic intramural nerve stimulation were closely mimicked by ATP, but not by acetylcholine or histamine. 7 Adenosine and dipyridamole reduced the contractions to both ATP and non-cholinergic nerve stimulation. 8 2-2′-Pyridylisatogen was not a specific blocker of either ATP or intramural nerve stimulation in the guinea-pig bladder. 2-Substituted imidazolines initiated spontaneous activity making it impossible to assess any blocking action that they may have had. 9 Prostaglandins (E1, E2 and F2α) gave weak, slow contractions and an increase in spontaneous activity. Both the response to ATP and non-adrenergic, non-cholinergic nerve stimulation were greatly potentiated in the presence of prostaglandins. 10 In the presence of indomethacin the response to non-adrenergic, non-cholinergic nerve stimulation was virtually abolished following desensitization to ATP. Images