Boronate-Affinity Glycan-Oriented Surface Imprinting: A New Strategy to Mimic Lectins for the Recognition of an Intact Glycoprotein and Its Characteristic Fragments
Boronate-Affinity Glycan-Oriented Surface Imprinting: A New Strategy to Mimic Lectins for the Recognition of an Intact Glycoprotein and Its Characteristic Fragments
复制标题
硼酸亲和聚糖导向的表面印迹：模拟凝集素识别完整糖蛋白及其特征片段的新策略
DOI：
10.1002/anie.201503066
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发表时间：
2015-08-24
影响因子：
16.6
通讯作者：
Liu, Zhen
中科院分区：
文献类型：
--
作者：
Bie, Zijun;Chen, Yang;Liu, Zhen
凝集素具有独特的结合性质，在分子识别方面具有特殊的价值。然而，凝集素有几个缺点，如难以制备和表现出较差的储存稳定性。硼酸盐亲和糖基表面印迹是制备凝集素类分子印迹聚合物的一种新策略。制备的分子印迹聚合物可以特异性识别完整的糖蛋白及其特征片段，即使在复杂的样品基质中也是如此。因此，在蛋白质组学、糖组学和诊断学等重要应用中，糖印迹分子印迹聚合物可能被证明是一种强大的工具。
Lectins possess unique binding properties and are of particular value in molecular recognition. However, lectins suffer from several disadvantages, such as being hard to prepare and showing poor storage stability. Boronate-affinity glycan- oriented surface imprinting was developed as a new strategy for the preparation of lectin-like molecularly imprinted polymers (MIPs). The prepared MIPs could specifically recognize an intact glycoprotein and its characteristic fragments, even within a complex sample matrix. Glycan-imprinted MIPs could thus prove to be powerful tools for important applications such as proteomics, glycomics, and diagnostics.