Oligosaccharides increased both leaf biomass and steviol glycosides content of Stevia rebaudiana
Oligosaccharides increased both leaf biomass and steviol glycosides content of Stevia rebaudiana
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DOI：
10.1016/j.plaphy.2023.107937
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发表时间：
2023-08-09
影响因子：
6.5
通讯作者：
Yin，Heng
中科院分区：
文献类型：
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作者：
Wang，Yu;Xu，Peiyu;Yin，Heng
甜菊醇糖苷（SGs）是一种重要的天然甜味剂。它们比不含卡路里的蔗糖甜200-350倍。目前，它们的生产仍然主要依赖于从甜菊糖（Stevia rebaudiana Bertoni）中提取。低聚糖是促进植物生长和次生代谢产物积累的环境友好型激发子。本研究将不同浓度的壳聚糖（COS）和海藻酸盐低聚糖（AOS）应用于甜菊糖中，探讨其对甜菊糖生长和SGs生物合成的影响。结果表明，黄芪多糖和黄芪多糖均通过增加叶片数和提高光合效率来促进生物量的产生，这可能与黄芪多糖降低脱落酸含量有关。50 mg/L AOS处理显著提高了甜叶菊苷（STV）和雷鲍迪苷A （Reb A）的含量，也显著提高了一些重要的少数苷，如Reb E、Reb D和Reb m的含量。SGs含量的增加是由于gc、UGT74G1、UGT85C2和UGT91D2等SGs生物合成相关基因表达量的增加所致。COS和AOS诱导甜叶菊茎秆的几何形状变化可能有助于提高其抗倒伏能力。因此，COS和AOS可用于甜叶菊的田间种植，以提高SGs的工业产量。
Steviol glycosides (SGs) are a variety of important natural sweeteners. They are 200–350 times sweeter than sucrose without calories. Currently, their production is still mainly dependent on extraction from Stevia rebaudiana Bertoni (stevia). Oligosaccharides are environmentally friendly elicitors that promote plant growth and accumulation of secondary metabolites. In the present study, different concentrations of chitosan oligosaccharides (COS) and alginate oligosaccharides (AOS) were applied to stevia to explore their effect on growth and SGs biosynthesis. It was found that both COS and AOS promoted biomass production by increasing the leaf number and photosynthetic efficiency, which may be related to the decreased content of abscisic acid. The content of SGs was significantly increased after 50 mg/L AOS treatment, which not only increased the contents of stevioside (STV) and rebaudioside A (Reb A) significantly, but some important minority glucosides, like Reb E, Reb D, and Reb M. The increased SGs contents were the combined effect of the higher expression of SGs biosynthesis related genes, including KAH, UGT74G1, UGT85C2, and UGT91D2. The geometry changes of stem induced by COS and AOS may help to increase the lodging resistance of stevia. Thus, COS and AOS can be used in the field planting of stevia to increase the yield of SGs for industrial purposes.