The miR156b-GmSPL9d module modulates nodulation by targeting multiple core nodulation genes in soybean.
The miR156b-GmSPL9d module modulates nodulation by targeting multiple core nodulation genes in soybean.
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DOI：
10.1111/nph.17899
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发表时间：
2022-03
期刊：
影响因子：
9.4
通讯作者：
Li, Xia
中科院分区：
文献类型：
--
作者：
Yun, Jinxia;Sun, Zhengxi;Jiang, Qiong;Wang, Youning;Wang, Can;Luo, Yuanqing;Zhang, Fengrong;Li, Xia
豆科植物的根在低氮和根瘤菌信号分子的作用下开始共生，并受到一个复杂的调节网络的动态调节，该网络协调根瘤菌感染和根瘤器官发生。已经表明miR 156 ‐SPL模块介导豆类中的结瘤;然而，该模块在结瘤过程中如何发挥其功能的决定性证据仍然难以捉摸。在这里，我们报告了miR 156 b-GmSPL 9d模块通过靶向大豆共生信号通路中的多个关键调控基因来调节共生共生。miR 156家族成员在大豆成熟过程中差异表达，miR 156 b主要通过靶向大豆SQUAMOSA启动子结合蛋白样9 d（GmSPL 9d）（大豆成熟的正调节因子）负调节大豆成熟。GmSPL 9d直接与miR 172 c启动子结合并激活其表达，表明GmSPL 9d的保守作用。此外，GmSPL 9d在根瘤形成和发育过程中与大豆根瘤形成标记基因nodule inception a（GmNINa）和GmENOD 40 - 1共表达。有趣的是，GmSPL 9d可以结合GmNINa和GmENOD 40 - 1的启动子并调节其表达。我们的数据表明，miR 156 b-GmSPL 9d模块作为大豆成熟的上游主调节因子，其协调参与大豆成熟的多个标记基因。
Symbiotic nodulation is initiated in the roots of legumes in response to low nitrogen and rhizobial signal molecules and is dynamically regulated by a complex regulatory network that coordinates rhizobial infection and nodule organogenesis. It has been shown that the miR156‐SPL module mediates nodulation in legumes; however, conclusive evidence of how this module exerts its function during nodulation remains elusive. Here, we report that the miR156b‐GmSPL9d module regulates symbiotic nodulation by targeting multiple key regulatory genes in the nodulation signalling pathway of soybean. miR156 family members are differentially expressed during nodulation, and miR156b negatively regulates nodulation by mainly targeting soybean SQUAMOSA promoter‐binding protein‐like 9d (GmSPL9d), a positive regulator of soybean nodulation. GmSPL9d directly binds to the miR172c promoter and activates its expression, suggesting a conserved role of GmSPL9d. Furthermore, GmSPL9d was coexpressed with the soybean nodulation marker genes nodule inception a (GmNINa) and GmENOD40‐1 during nodule formation and development. Intriguingly, GmSPL9d can bind to the promoters of GmNINa and GmENOD40‐1 and regulate their expression. Our data demonstrate that the miR156b‐GmSPL9d module acts as an upstream master regulator of soybean nodulation, which coordinates multiple marker genes involved in soybean nodulation.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
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影响因子：
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影响因子：
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作者：
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通讯作者：
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