Extent of terminal complementarity modulates the balance between transcription and replication of vesicular stomatitis virus RNA.
Extent of terminal complementarity modulates the balance between transcription and replication of vesicular stomatitis virus RNA.
复制标题
末端互补性的程度调节水泡性口炎病毒RNA的转录和复制之间的平衡。
DOI：
10.1073/pnas.91.18.8587
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发表时间：
1994
影响因子：
11.1
通讯作者：
Ball,LA
中科院分区：
文献类型：
--
作者：
Wertz,GW;Whelan,S;LeGrone,A;Ball,LA
我们将包含水疱性口炎病毒基因组真实 5' 和 3' 末端的亚基因组 RNA 的模板特性与野生型末端经过工程改造以将其互补性从 8 个核苷酸延伸到 51 个核苷酸的 RNA 的模板特性进行了比较，如缺陷干扰 RNA 中所见。具有真实 5' 和 3' 末端的 RNA 可以指导大量转录，但复制量较低。相比之下，来自基因组任一端的具有互补末端的RNA复制良好，但转录较差或根本不转录。这些结果对于理解 RNA 复制和转录的机制具有重要意义；他们解释了有缺陷的干扰RNA的复制优势，并证明末端互补性的程度而不是其确切序列是模板是否主要指导转录或复制的主要决定因素。
We compared the template properties of a subgenomic RNA that contained the authentic 5' and 3' ends of the vesicular stomatitis virus genome with those of RNAs in which the wild-type termini were engineered to extend their complementarity from 8 to 51 nucleotides as seen in defective interfering RNAs. The RNA with authentic 5' and 3' ends directed abundant transcription but low replication. In contrast, RNAs with complementary termini derived from either end of the genome replicated well but transcribed poorly or not at all. These results have implications for understanding the mechanisms of RNA replication and transcription; they explain the replicative dominance of defective interfering RNAs and demonstrate that the extent of terminal complementarity rather than its exact sequence is a major determinant of whether the template predominantly directs transcription or replication.