Complementary action of jasmonic acid on salicylic acid in mediating fungal elicitor-induced flavonol glycoside accumulation of Ginkgo biloba cells.
Complementary action of jasmonic acid on salicylic acid in mediating fungal elicitor-induced flavonol glycoside accumulation of Ginkgo biloba cells.
复制标题
DOI：
10.1111/j.1365-3040.2009.01976.x
复制
发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Maojun Xu;Jufang Dong;Huizhong Wang;Luqi Huang
中科院分区：
文献类型：
--
作者：
Maojun Xu;Jufang Dong;Huizhong Wang;Luqi Huang
茉莉酸(JA)和水杨酸(SA)在植物防御反应中的拮抗作用已被充分证实。然而，它们在次生代谢物产生中的关系在很大程度上是未知的。在这里，我们报道了来自棉疫霉的蛋白激发子PB90触发了银杏细胞JA的产生、SA的积累和黄酮苷的产生。JA抑制剂不仅抑制PB90触发的JA的产生，而且还抑制激发子诱导的黄酮苷的产生。然而，即使JA的产生被完全抑制，激发子仍能促进黄酮醇糖苷的产生。当JA信号被抑制时，SA水解酶基因NaHG的过表达不仅可以消除SA的积累，还可以抑制激发子诱导的黄酮苷的产生。有趣的是，在没有JA抑制剂的情况下，NahG的表达并不抑制激发子诱导的黄酮苷积累。此外，当转基因细胞中SA的积累受到损害时，JA水平显著增加。总之，这些数据表明JA和SA都参与了PB90诱导的黄酮苷的产生。此外，我们还证明，当SA信号受损时，JA信号可能被增强以替代SA来介导激发子诱导的黄酮苷积累，这揭示了JA和SA在调节植物次生代谢产物产生方面的不寻常的互补关系。
The antagonistic action between jasmonic acid (JA) and salicylic acid (SA) in plant defence responses has been well documented. However, their relationship in secondary metabolite production is largely unknown. Here, we report that PB90, a protein elicitor from Phytophthora boehmeriae, triggers JA generation, SA accumulation and flavonol glycoside production of Ginkgo biloba cells. JA inhibitors suppress not only PB90-triggered JA generation, but also the elicitor-induced flavonol glycoside production. However, the elicitor can still enhance flavonol glycoside production even though the JA generation is totally inhibited. Over-expression of SA hydrolase gene NahG not only abolishes SA accumulation, but also suppresses the elicitor-induced flavonol glycoside production when JA signalling is inhibited. Interestingly, expression of NahG does not inhibit the elicitor-induced flavonol glycoside accumulation in the absence of JA inhibitors. Moreover, JA levels are significantly enhanced when SA accumulation is impaired in the transgenic cells. Together, the data suggest that both JA and SA are involved in PB90-induced flavonol glycoside production. Furthermore, we demonstrate that JA signalling might be enhanced to substitute for SA to mediate the elicitor-induced flavonol glycoside accumulation when SA signalling is impaired, which reveals an unusual complementary relationship between JA and SA in mediating plant secondary metabolite production.