Binding of polystyrene and carbon black nanoparticles to blood serum proteins
Binding of polystyrene and carbon black nanoparticles to blood serum proteins
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DOI：
10.3109/08958378.2011.583944
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发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Kreyling, Wolfgang G.
中科院分区：
文献类型：
--
作者：
Fertsch-Gapp, Stefanie;Semmler-Behnke, Manuela;Kreyling, Wolfgang G.
目的：在本研究中，我们研究影响 NP 与蛋白质之间结合的参数。材料和方法：我们使用蛋白质白蛋白、转铁蛋白 (TF) 和载脂蛋白 A-1（均称为来自 ELF 的蛋白质）和不同的 NP（具有负电、正电和中性表面涂层的聚苯乙烯 NP，Printex G 和 Printex 90）作为模型。结果：在所有情况下，发现并描述了添加的 NP 量与结合蛋白量之间的线性相关性通过结合指数定量。牛血清白蛋白 (BSA)、TF 和 apo A-1 与疏水性 NP 的结合程度最大，这表明 NP 的表面特性非常重要。讨论：结合指数表明初级颗粒尺寸和表面特性（包括疏水性）的相关性。结论：NP 的尺寸和表面修饰决定了它们的蛋白质结合。我们的结果表明，BSA、TF 和 Apo A-1 与 NP 形成的缀合物可能在它们跨越气血屏障的易位和随后的生物动力学中发挥重要作用。
Objective: In this study, we investigate the parameters that influence the binding between NP and proteins.Materials and methods: We used the proteins albumin, transferrin (TF), and apolipoprotein A-1 (all known as proteins from ELF) and different NP (polystyrene NP with negative, positive, and neutral surface coatings, Printex G and Printex 90) as models.Results: In all cases, a linear correlation of the added NP amount and the amount of bound proteins was found and was described quantitatively by binding indices. Bovine serum albumin (BSA), TF, and apo A-1 were bound to the largest extent to hydrophobic NP, which shows the extraordinary importance of the NP''s surface properties.Discussion: The binding index indicates the relevance of primary particle size and surface properties, including hydrophobicity.Conclusion: Size and surface modifications of NP determine their protein binding. Our results suggest that the formation of conjugates of BSA, TF, and Apo A-1 with NP may play an important role in their translocation across the air--blood--barrier and subsequent biokinetics.