Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography
Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography
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DOI：
10.1016/j.chroma.2012.12.037
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发表时间：
2013-02-08
影响因子：
4.1
通讯作者：
McCalley, David V.
中科院分区：
文献类型：
--
作者：
Kumar, Abhinav;Heaton, James C.;McCalley, David V.
一系列的29种化合物，包括酸，碱和中性的保留数据，获得6个不同的亲水相互作用（HILIC）柱，包括裸硅胶，两性离子和那些与中性键合配体键合。这项工作的主要目的是评估各种实验变量的影响，如固定相的性质，缓冲液pH值，缓冲液浓度，有机溶剂及其浓度，温度，以确定哪些因素对分离的选择性影响最大。还考虑了溶质性质对分离的影响，如log D值。希望通过这种方式提供一个实用指南，帮助选择在HILIC实现分离的条件。固定相的性质被认为对选择性有最大的影响。(C)2012爱思唯尔有限公司版权所有。
Retention data for a series of 29 compounds comprising acids, bases and neutrals were obtained on six different hydrophilic interaction (HILIC) columns including bare silica, zwitterionic and those bonded with neutral bonded ligands. The principal aim of the work was to evaluate the effect of various experimental variables such as the nature of the stationary phase, buffer pH, buffer concentration, organic solvent and its concentration, and temperature, in order to determine which factors gave the most effect on the selectivity of the separation. The influence of solute properties on the separation, such as log D values, was also considered. In this way, it was hoped to provide a practical guide to aid in the selection of conditions to achieve a separation in HILIC. The nature of the stationary phase was found to have the greatest effect on selectivity. (C) 2012 Elsevier B.V. All rights reserved.