Antisense transcription regulates the expression of the enterohemorrhagic Escherichia coli virulence regulatory gene ler in response to the intracellular iron concentration.
Antisense transcription regulates the expression of the enterohemorrhagic Escherichia coli virulence regulatory gene ler in response to the intracellular iron concentration.
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DOI：
10.1371/journal.pone.0101582
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Oshima T
中科院分区：
文献类型：
--
作者：
Tobe T;Yen H;Takahashi H;Kagayama Y;Ogasawara N;Oshima T
肠出血性大肠杆菌（EHEC） O157:H7等肠道病原体在其生命周期中会遇到不同浓度的铁。在胃肠道中，由于宿主因素的吸收，可用的游离铁的量是有限的。肠出血性大肠杆菌和其他肠道病原体已经发展出复杂的铁反应系统来利用有限的铁资源，这些系统主要由Fur抑制蛋白调节。铁浓度可能是控制肠道基因表达的信号。在这项研究中，我们探讨了铁在肠出血性大肠杆菌LEE（肠细胞清除位点）毒力基因表达中的作用。与fur调控基因的表达相反，无论铁浓度如何，LEE基因的表达在毛皮突变体中都大大降低。作为lee编码的主调控因子，ler基因的表达在转录后阶段受到皮毛突变的影响。进一步分析表明，Fur的缺失通过增加细胞内游离铁的浓度影响了ler基因的翻译，反义链的转录是调控的必要条件。结果表明，LEE基因的表达与细胞内游离铁稳态的控制密切相关。
Enteric pathogens, such as enterohemorrhagic E. coli (EHEC) O157:H7, encounter varying concentrations of iron during their life cycle. In the gastrointestinal tract, the amount of available free iron is limited because of absorption by host factors. EHEC and other enteric pathogens have developed sophisticated iron-responsive systems to utilize limited iron resources, and these systems are primarily regulated by the Fur repressor protein. The iron concentration could be a signal that controls gene expression in the intestines. In this study, we explored the role of iron in LEE (locus for enterocyte effacement) virulence gene expression in EHEC. In contrast to the expression of Fur-regulated genes, the expression of LEE genes was greatly reduced in fur mutants irrespective of the iron concentration. The expression of the ler gene, the LEE-encoded master regulator, was affected at a post-transcription step by fur mutation. Further analysis showed that the loss of Fur affected the translation of the ler gene by increasing the intracellular concentration of free iron, and the transcription of the antisense strand was necessary for regulation. The results indicate that LEE gene expression is closely linked to the control of intracellular free iron homeostasis.
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影响因子：
4.8
作者：
McHugh, JP;Rodríguez-Quiñones, F;Andrews, SC
通讯作者：
Andrews, SC
影响因子：
3.6
作者：
Nachin, L;El Hassouni, M;Barras, F
通讯作者：
Barras, F
影响因子：
2.8
作者：
Iyoda, S;Watanabe, H
通讯作者：
Watanabe, H
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
CALDERWOOD, SB;MEKALANOS, JJ
通讯作者：
MEKALANOS, JJ