Background bisphenol A in experimental materials and its implication to low-dose in vitro study
Background bisphenol A in experimental materials and its implication to low-dose in vitro study
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DOI：
10.1016/j.chemosphere.2010.07.048
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发表时间：
2010-10-01
期刊：
影响因子：
8.8
通讯作者：
Jin, Xiaolei
中科院分区：
文献类型：
--
作者：
Cao, Xu-Liang;Corriveau, Jeannette;Jin, Xiaolei
体外低剂量研究对于了解双酚 A (BPA) 的作用机制非常重要。目前体外研究中使用的 BPA 剂量差异很大，据报道剂量低至 10(-15) M。体外低剂量研究中使用的 BPA 实际剂量很少进行分析检查，应调查或考虑实验材料中的背景 BPA 水平，这将决定所使用的最低 BPA 剂量。在本研究中，对体外低剂量 BPA 研究中常用的各种材料的背景 BPA 水平进行了调查。使用一次性移液器和移液器吸头产生的背景 BPA 水平较低（< 0.20 ng mL(-1) 或 0.88 nM）。还在多种商业缓冲溶液中检测到 BPA，其水平接近定量方法限 (LOQ) (0.02 ng mL(-1)：0.088 nM）。然而，在从不同来源获得的所有细胞培养基中均检测到双酚 A，其含量范围为 0.080 至 4.26 ng mL(-1)（或 0.35 至 19 nM），平均为 0.83 ng mL(-1) (3.5 nM)。我们建议用于低剂量 BPA 研究的培养基应在使用前分析背景 BPA 水平，并应使用 BPA 水平最低的培养基。 Crown 版权所有 (c) 2010 由 Elsevier Ltd 出版。保留所有权利。
In vitro low-dose studies are important to understand the mechanisms of bisphenol A (BPA) action. BPA doses used in current in vitro studies varied considerably, and doses as low as 10(-15) M have been reported. The actual doses of BPA used in the in vitro low-dose studies were rarely checked analytically, and the background BPA levels in experimental materials, which will determine the lowest BPA dose to be used, should be investigated or considered. In this study, the background BPA levels in various materials typically used in in vitro low-dose studies for BPA were investigated. Background BPA levels from the use of disposable pipettes and pipette tips were low (< 0.20 ng mL(-1) or 0.88 nM). BPA was also detected in several commercial buffer solutions at levels close to the method limit of quantification (LOQ) (0.02 ng mL(-1): 0.088 nM). However, BPA was detected in all cell culture media obtained from various sources at levels ranging from 0.080 to 4.26 ng mL(-1) (or 0.35 to 19 nM) with an average of 0.83 ng mL(-1) (3.5 nM). We suggest that culture media used for low-dose BPA studies should be analysed for background BPA levels prior to use, and the medium with the lowest BPA levels should be used. Crown Copyright (c) 2010 Published by Elsevier Ltd. All rights reserved.