Epidemiology and Evolution of Emerging Porcine Circovirus-like Viruses in Pigs with Hemorrhagic Dysentery and Diarrhea Symptoms in Central China from 2018 to 2021.
Epidemiology and Evolution of Emerging Porcine Circovirus-like Viruses in Pigs with Hemorrhagic Dysentery and Diarrhea Symptoms in Central China from 2018 to 2021.
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DOI：
10.3390/v13112282
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发表时间：
2021-11-15
期刊：
影响因子：
--
通讯作者：
Tu J
中科院分区：
文献类型：
--
作者：
Yang K;Zhang M;Liu Q;Cao Y;Zhang W;Liang Y;Song X;Ji K;Shao Y;Qi K;Tu J
猪圆环病毒样病毒 (PCLV) 是一种编码环状 Rep 的单链 DNA 病毒，可能与猪腹泻症状的发生有关。在本研究中，我们回顾性分析了中国安徽三年来的病例，报告了一例可能由 PCLV 引起的妊娠母猪出血性肠炎和死亡病例。此外，我们分析了PCLV的进化特征，发现突变、重组和选择压力在PCLV的进化中都发挥了重要作用。我们在两个不同的分支中鉴定了 N15D 和 T17S 以及 L56T、T58R、K59Q、M62R、L75I 和 R190K 突变，并且我们注意到一组中国菌株 Rep 中的重组事件。选择压力分析显示，PCLV获得了更多的正选择，表明病毒处于持续进化状态。 PR2图、ENC图和中性分析表明，在密码子使用模式的形成中，自然选择的作用比突变压力的作用更大。该研究首次在出血性痢疾和死亡母猪中鉴定出PCLV，为中国猪PCLV感染提供了新的流行病学信息。
Porcine circovirus-like virus (PCLV) is a type of circular Rep-encoding single-stranded DNA virus and may be associated with the development of diarrheal symptoms in pigs. In this study, we retrospectively analyzed three years of past cases in Anhui, China, and reported a case of hemorrhagic enteritis and death in a pregnant sow possibly caused by PCLV. In addition, we analyzed the evolutionary characteristics of PCLV and found that mutation, recombination and selective pressure all played an important role in the evolution of PCLV. We identified N15D and T17S as well as L56T, T58R, K59Q, M62R, L75I and R190K mutations in two different branches, and we noted recombination events in the Rep of a group of Chinese strains. Analysis of selection pressure revealed that PCLV gained more positive selection, indicating that the virus is in a continuous evolutionary state. The PR2 plot, ENC-plot and neutrality analysis showed a greater role of natural selection than that of mutational pressure in the formation of codon usage patterns. This study is the first to identify PCLV in sows with hemorrhagic dysentery and death, and it provides new epidemiological information on PCLV infection in pigs in China.
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