Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression
Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression
复制标题
硒代蛋氨酸通过上调 SelW 表达抑制 CYP450 1A5 表达，减轻 AFB1 诱导的原代鸡肝细胞损伤
DOI：
10.1021/acs.jafc.6b05308
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发表时间：
2017-03-29
影响因子：
6.1
通讯作者：
Huang, Kehe
中科院分区：
文献类型：
--
作者：
Chen, Xingxiang;Che, Chaoping;Huang, Kehe
本研究旨在评估硒代蛋氨酸 (SeMet) 对黄曲霉毒素 B1 (AFB1) 诱导的原代鸡肝细胞肝毒性的保护作用。细胞活力和乳酸脱氢酶活性测定揭示了 AFB1 对鸡肝细胞毒性的剂量依赖性。 AFB1 浓度>.05 mu g/mL 显着降低谷胱甘肽和总超氧化物歧化酶水平，并增加丙二醛浓度和细胞色素P450 酶1A5 (CYP450 1A5) mRNA 水平(P < 0.05)。然而，AFB1；不影响 CYP450 3A37 mRNA 水平。补充 2 μM SeMet 可防止 AFB1 诱导的变化，并显着增加硒蛋白 W (SelW) mRNA 水平 (P < 0.05)。此外，SelW 敲低减弱了 SeMet 对 AFB1 诱导损伤的保护作用，并显着增加了 CYP450 1A5 表达水平（P < 0.05）。因此，SeMet通过提高SelW表达来减轻AFB1诱导的鸡原代肝细胞损伤，从而抑制CYP450 1A5表达。
This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell-viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >.05 mu g/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however; did not affect CYP450 3A37 mRNA levels. Supplementation with 2 mu M SeMet protected against AFB1 induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in priinary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.