Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea
Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea
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DOI：
10.1007/s00705-013-1770-z
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发表时间：
2013-08-01
影响因子：
2.7
通讯作者：
Lee, Changhee
中科院分区：
文献类型：
--
作者：
Lee, Yu Na;Lee, Changhee
利用猪肺泡巨噬细胞连续培养，从仔猪肺组织中分离到一种新的致细胞病变副粘病毒。形态学和遗传学研究表明，这种猪病毒（pPIV 5）属于副粘病毒科副流感病毒5型。我们试图确定第一个韩国pPIV 5分离株（命名为KNU-11）的完整核苷酸序列。KNU-11基因组全长15，246个核苷酸，由7个非重叠基因（3 ′-N-V/P-M-F-SH-HN-L-5 ′）组成，编码8种蛋白质。KNU-11和PIV 5（原猴病毒5，SV 5）（一种原型副粘病毒）之间的核苷酸序列同一性总体程度为98.7%，推定蛋白质与PIV 5具有74.4%至99.2%的氨基酸同一性。系统发育分析进一步证明，新型pPIV 5分离株是副粘病毒亚科Rubulavirus属的成员。本研究描述了韩国pPIV 5分离株的鉴定和基因组特征。
A novel cytopathogenic paramyxovirus was isolated from a lung sample from a piglet, using continuous porcine alveolar macrophage cells. Morphologic and genetic studies indicated that this porcine virus (pPIV5) belongs to the species Parainfluenza 5 in the family Paramyxoviridae. We attempted to determine the complete nucleotide sequence of the first Korean pPIV5 isolate, designated KNU-11. The full-length genome of KNU-11 was found to be 15,246 nucleotides in length and consist of seven nonoverlapping genes (3'-N-V/P-M-F-SH-HN-L-5') predicted to encode eight proteins. The overall degree of nucleotide sequence identity was 98.7 % between KNU-11 and PIV5 (formerly simian virus 5, SV5), a prototype paramyxovirus, and the putative proteins had 74.4 to 99.2 % amino acid identity to those of PIV5. Phylogenetic analysis further demonstrated that the novel pPIV5 isolate is a member of the genus Rubulavirus of the subfamily Paramyxovirinae. The present study describes the identification and genomic characterization of a pPIV5 isolate in South Korea.