BONE-MARROW STROMAL CELL-LINES WITH LYMPHOPOIETIC ACTIVITY EXPRESS HIGH-LEVELS OF A PRE-B NEOPLASIA-ASSOCIATED MOLECULE
BONE-MARROW STROMAL CELL-LINES WITH LYMPHOPOIETIC ACTIVITY EXPRESS HIGH-LEVELS OF A PRE-B NEOPLASIA-ASSOCIATED MOLECULE
复制标题
DOI：
10.1016/0092-8674(87)90709-4
复制
发表时间：
1987-03-27
期刊：
影响因子：
64.5
通讯作者：
WEISSMAN, IL
中科院分区：
文献类型：
--
作者：
WHITLOCK, CA;TIDMARSH, GF;WEISSMAN, IL
骨髓基质细胞系已被分离出来，在体外直接支持B淋巴细胞生成。单个B系前体细胞在某些基质细胞系上增殖和分化，以建立长期的B系培养物。这些淋巴基质细胞产生新的可溶性因子，支持体外建立的前B细胞群的增殖。在淋巴细胞生成基质细胞系上建立的类淋巴细胞群体缺乏表面Ig-承载细胞，但当转移到混合骨髓饲养层时产生表面IG+细胞。几种基质细胞系表达由单克隆抗体MAb 6C 3检测到的B系肿瘤标记物。值得注意的是，只有6C 3Aghi基质系支持B系细胞的长期增殖。我们建议，6C 3抗原承载分子可能在基质细胞依赖性，前B细胞增殖，以及在前B白血病的肿瘤增殖中发挥作用。
Bone marrow stroma cell lines have been isolated that directly support B lymphoiesis in vitro. Single B-lineage precursors proliferate and differentiate on certain of these stroma cell lines to establish long-term B-lineage cultures. These lymphopoietic stroma cells produce novel soluble factors that support proliferation of in vitro established pre-B cell populations. Lymphoid populations established on lymphopoietic stromal cell lines lack surface Ig-bearing cells, but give rise to surface Ig+ cells when transferred to mixed bone marrow feeder layers. Several stromal lines expressed a B-lineage neoplasia marker detected by the monoclonal antibody MAb6C3. Remarkably, only the 6C3Aghi stroma lines supported long-term proliferation of B-lineage cells. We propose that the 6C3 antigen-bearing molecule may play a role in stroma cell-dependent, pre-B cell proliferation, as well as in neoplastic proliferation of pre-B leukemias.