Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters
Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters
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DOI：
10.1021/acs.analchem.5b00468
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发表时间：
2015-05-05
影响因子：
7.4
通讯作者：
Wang, Erkang
中科院分区：
文献类型：
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作者：
Teng, Ye;Jia, Xiaofang;Wang, Erkang
在这项工作中，建立了一个灵敏且选择性的比率荧光传感平台，用于使用谷胱甘肽（GSH）保护的金纳米簇（Au NC）作为探针检测酪氨酸酶（TYR）活性和多巴胺（DA）。在 350 nm 激发下，Au NCs 显示出强烈的红色发射，可以被醌有效猝灭。 TYR是一种典型的多酚氧化酶，可以催化DA氧化为邻醌，从而猝灭Au NCs的荧光。此外，TYR和DA的反应在400 nm处产生发射带，该发射带以TYR/DA浓度依赖性方式增加。利用比例信号变化可轻松、灵敏且选择性地检测 TYR 活性和 DA。 TYR活性的线性范围为0.006-3.6单位mL(-1)，而DA检测的线性范围为1.0 nM至1.0 mM。此外，它还为生物医学研究中的 TYR 抑制剂筛选构建了一个有用的平台。
In this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (TYR) activity and dopamine (DA) using glutathione (GSH) protected gold nanoclusters (Au NCs) as probes. Upon excitation at 350 nm, Au NCs displayed an intense red emission, which could be effectively quenched by quinones. TYR, a typical polyphenol oxidase, can catalyze the oxidization of DA to o-quinone and therefore quenched the fluorescence of Au NCs. Moreover, the reaction of TYR and DA gave rise to an emission band at 400 nm, which increased in a TYR/DA-concentration-dependent manner. The ratiometric signal variations were utilized for facile, sensitive, and selective detection of TYR activity and DA. A linear range was obtained from 0.006-3.6 unit mL(-1) of TYR activity, while the linear range for detection of DA was 1.0 nM to 1.0 mM. Additionally, it constructed a useful platform for TYR inhibitor screening in biomedical research.