Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility.
Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility.
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基于氧化纤维素硫酸盐和羧甲基壳聚糖的水凝胶：凝胶固有特性、稳定性和生物相容性研究
DOI：
10.1002/mabi.202100098
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发表时间：
2021
影响因子：
4.6
通讯作者：
T. Groth
中科院分区：
文献类型：
--
作者：
C. Willems;M.-L. Trutschel;V. Mazaikina;J. Strätz;K. Mäder;S. Fischer;T. Groth
纤维素和壳聚糖是制造生物活性支架的优异成分，因为它们具有生物相容性并且来源丰富。它们的衍生物O羧甲基壳聚糖（CMChi）和氧化纤维素硫酸盐（oxCS）由于形成亚胺键进行交联，可以形成原位胶凝、生物活性水凝胶。这里研究了硫酸化度 (DS)、氧化度 (DO) 和 oxCS 的分子量对此类水凝胶的内在和流变特性及其支持人类脂肪干细胞 (hADSC) 存活和生长的能力的影响。研究发现，水凝胶的pH值通常呈弱酸性，而其网络密度和弹性模量取决于DS和DO，这使得水凝胶的性能可调。大量研究表明，水凝胶可以稳定长达 14 天，并且其稳定性在很大程度上取决于 DO、分子量和组分混合比。对 hADSC 水凝胶的细胞毒性研究表明，生物相容性凝胶取决于分子量和氧化程度，对活细胞的作用长达 14 天。这些发现有助于开发专门用于组织工程应用的水凝胶，以替代不同类型的结缔组织。
Cellulose and chitosan are excellent components for the fabrication of bioactive scaffolds, as they are biocompatible and abundantly available. Their derivativesOcarboxymethyl chitosan (CMChi) and oxidized cellulose sulfate (oxCS) can form in situ gelling, bioactive hydrogels, due to the formation of imine bonds for crosslinking. Here the influence of the degrees of sulfation (DS), oxidation (DO), and the molecular weight of oxCS on intrinsic and rheological properties of such hydrogels and their ability to support the survival and growth of human‐adipose‐derived stem cells (hADSC) is investigated. It is found that the pH of the hydrogels is generally slightly acidic, while their network density and E‐modulus are found to be dependent on the DS and DO, which makes the properties of hydrogels tunable. Extensive studies show that hydrogels can be stable for up to 14 days and that their stability is largely dependent on the DO, molecular weight, and the components mixing ratio. Cytotoxicity studies of the hydrogel with hADSCs show biocompatible gels in dependence on the molecular weight and degree of oxidation with viable cells up to 14 days. These findings can help to develop specifically tailored hydrogels for tissue engineering applications to replace different types of connective tissue.