OsTCP19 coordinates inhibition of lignin biosynthesis and promotion of cellulose biosynthesis to modify lodging resistance in rice
OsTCP19 coordinates inhibition of lignin biosynthesis and promotion of cellulose biosynthesis to modify lodging resistance in rice
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DOI：
10.1093/jxb/erad367
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发表时间：
2023-09-19
影响因子：
6.9
通讯作者：
Gui，Jinshan
中科院分区：
文献类型：
--
作者：
Lv，Siwei;Lin，Zengshun;Gui，Jinshan
木质素和纤维素是植物次生细胞壁的两种基本元素，它们塑造了茎的机械特性，以防止作物倒伏。然而，木质素和纤维素复合材料的组合调节以实现最佳力学特性的机制尚不清楚。在此，我们发现OsTCP19的高表达协同抑制了木质素的生物合成，促进了纤维素的生物合成，从而增强了抗倒性。而OsTCP19则相反，协同激活木质素的生物合成，抑制纤维素的生物合成，导致倒伏敏感性。我们发现OsTCP19可以与MYB108和MYB103L的启动子结合来激活它们的表达。MYB108和MYB103L分别负责抑制木质素生物合成和促进纤维素生物合成。此外，OsTCP19在纤维中的上调还提高了籽粒产量和抗倒伏性。本研究确定OsTCP19-OsMYB108/OsMYB103L模块是水稻木质素和纤维素产生的关键调控因子。这一发现为精确操纵木质素-纤维素组成以提高秸秆抗倒伏的力学性能开辟了一条新的途径。
Lignin and cellulose are two essential elements of plant secondary cell walls that shape the mechanical characteristics of the culm to prevent crop lodging. However, the mechanisms of combinatorial regulation of lignin and cellulose composites to achieve optimal mechanical characteristics are unclear. Here, we showed that increasing OsTCP19 expression coordinately repressed lignin biosynthesis and promoted cellulose biosynthesis, resulting in enhanced lodging resistance. Repression of OsTCP19, on the contrary, coordinately activated lignin biosynthesis and inhibited cellulose biosynthesis, leading to lodging susceptibility. We revealed that OsTCP19 binds to the promoters of both MYB108 and MYB103L to activate their expression. MYB108 and MYB103L were responsible for repressing lignin biosynthesis and promoting cellulose biosynthesis, respectively. Moreover, up-regulation of OsTCP19 in fibers improved grain yield and lodging resistance. This study identified the OsTCP19-OsMYB108/OsMYB103L module as a key regulator of lignin and cellulose production in rice. The discovery opens up a new avenue for precisely manipulating lignin-cellulose composition to improve culm mechanical properties for lodging resistance.