cis- and trans-acting transcriptional regulation of visna virus.
cis- and trans-acting transcriptional regulation of visna virus.
复制标题
维斯纳病毒的顺式和反式转录调控。
DOI：
10.1126/science.2990051
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Narayan,O
中科院分区：
文献类型：
--
作者：
Hess,JL;Clements,JE;Narayan,O
维斯纳病毒（Visna virus）是绵羊的一种致病性慢病毒，与人类嗜T淋巴细胞病毒III型（HTLV-III）相关，HTLV-III可能是获得性免疫缺陷综合征（AIDS）的病原体。通过基于细菌基因氯霉素乙酰转移酶（CAT）的瞬时表达的测定来研究维斯纳病毒启动子和增强子序列的转录活性。结果表明，维斯纳病毒的高水平表达部分是由于给予病毒启动子高水平转录活性的顺式作用增强子序列。此外，位于表达CAT基因的质粒中的维斯纳病毒启动子的转录速率在感染的细胞中比未感染的细胞大得多。这种反式作用的转录激活现象可能涉及病毒或细胞编码的因子。
Visna virus is a pathogenic lentivirus of sheep that is related to human T-cell lymphotropic virus type III (HTLV-III), the probable etiologic agent of the acquired immune deficiency syndrome (AIDS). The transcriptional activity of visna virus promoter and enhancer sequences was studied by means of an assay based on the transient expression of the bacterial gene chloramphenicol acetyltransferase (CAT). The results suggest that the high level of expression of visna virus is due in part tocis-acting enhancer sequences that give the viral promoter a high level of transcriptional activity. In addition, the rate of transcription from the visna virus promoter situated in a plasmid expressing the CAT gene was much greater in infected than uninfected cells. This phenomenon of trans-acting transcriptional activation may involve either virally or cellularly encoded factors.