Expression and localization of the homeodomain-containing protein HEX in human thyroid tumors
Expression and localization of the homeodomain-containing protein HEX in human thyroid tumors
复制标题
DOI：
10.1210/jc.87.3.1376
复制
发表时间：
2002-03-01
影响因子：
5.8
通讯作者：
Damante, G
中科院分区：
文献类型：
--
作者：
D'Elia, AV;Tell, G;Damante, G
同源异型盒基因参与上皮和造血组织的肿瘤转化。趋异同源盒基因HEX在前内脏内胚层中表达。在小鼠发育早期和一些内胚层来源的成年组织中，包括肝脏和甲状腺。尽管已经提出了在白血病发生中的作用，但关于HEX参与人类上皮肿瘤的数据很少。在此，我们分析了HEX的表达和亚细胞定位在一系列的55人甲状腺肿瘤和几个肿瘤细胞系。RT-PCR检测HEX mRNA在正常组织或甲状腺腺瘤和分化（乳头状和滤泡状）癌。HEX mRNA在大多数未分化癌中也有表达。通过免疫组织化学方法研究HEX蛋白的亚细胞定位。在正常组织和腺瘤中，HEX蛋白表达于细胞核和细胞质中。与此相反，分化和未分化癌，以及肿瘤细胞系的调查，显示HEX蛋白只在细胞质中。这些发现表明，HEX进入甲状腺细胞核的调节可能是人类甲状腺肿瘤发生过程中的一个关键步骤。
Homeobox genes are involved in neoplastic transformation of both epithelial and hemopoietic tissues. The divergent homeobox gene HEX is expressed in the anterior visceral endoderm. during early mouse development and in some adult tissues of endodermal origin, including liver and thyroid. Whereas a role in leukemyogenesis has been proposed already, few data are available on the involvement of HEX in human epithelial tumors. Herein, we analyzed HEX expression and subcellular localization in a series of 55 human thyroid tumors and in several tumoral cell lines. HEX mRNA was detected by RT-PCR either in normal tissues or in thyroid adenomas and differentiated (papillary and follicular) carcinomas. HEX mRNA was also expressed in most undifferentiated carcinomas. Subcellular localization of HEX protein was investigated by immunohistochemistry. In normal tissues and adenomas, HEX protein was present both in nucleus and cytoplasm. In contrast, both differentiated and undifferentiated carcinomas, as well as the tumoral cell lines investigated, showed HEX protein only in the cytoplasm. These findings suggest that regulation of HEX entry in the nucleus of thyrocytes may represent a critical step during human thyroid tumorigenesis.